This video demonstrates how to use the NEBuilder® Assembly Tool to build a construct using a restriction enzyme digested vector and two PCR-generated inserts. The tool will help to design PCR primers containing the required overlap sequences. We will also regenerate one of the restriction enzyme recognition sites.
Tags DNA Modifying Enzymes and Cloning Technologies
Related Products Gibson Assembly® Master Mix, NEBuilder® HiFi DNA Assembly Master Mix, NEBuilder® HiFi DNA Assembly Bundle for Large Fragments, Gibson Assembly® Cloning Kit, NEBuilder® HiFi DNA Assembly Cloning Kit