Customers frequently ask “Why is there a white precipitate in my T4 DNA ligase buffer?”
If you see a white precipitate in your T4 DNA ligase buffer it is most likely DTT falling out of solution. It is important not to heat the buffer to resuspend the precipitate, as this will degrade the ATP, which is necessary for ligation. DTT is found in excess in the buffer and the small amount lost in the precipitation will not affect your ligation efficiency.
Remember, if you have any questions you can contact us at info@neb.com.
Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.
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