Quick Tips - What protocol adjustments can I make when purifying a large plasmid?

This video gives tips on adjustments you can make to your plasmid purification protocol when you're working with a large plasmid.

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Customers often ask what protocol adjustments should I make when purifying a large plasmid?

For plasmids that are larger than 10 kb heating the elution buffer to 50 degrees Celsius prior to adding it to the column can improve yields.

Remember if you have any additional questions you can always contact our tech support team at info@neb.com 

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