What is isothermal amplification?
Isothermal amplification is a way to get a lot of copies of a target sequence in a short period of time at a constant temperature.
How can you use restriction enzymes in isothermal amplification?
Isothermal amplification uses nicking enzymes to nick just a single strand, and then that nick gets translated by Bst polymerase to get an isothermal amplification of your target.
What is the future of restriction enzymes in isothermal amplification?
You can choose from a variety of nicking enzymes to detect your target of interest, and this gene detection will be useful in point of care diagnostics for a long time to come.
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