Home Resources Video Library Gene Editing 101: A practical guide to genome editing

Gene Editing 101: A practical guide to genome editing

In this webinar you will learn how to increase editing efficiency by directly introducing Cas9 ribonucleoproteins (RNPs) to cells through electroporation or lipofection. Rapid sgRNA synthesis requiring only a single user-supplied ~55mer single-stranded DNA oligonucleotide is described. Methods for assessing genome editing efficiency will be discussed including T7-endonuclease I-based methods, sequencing-based methods, and in vitro Cas9 digestion.

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Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.

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