DNA Sequences and Maps Tool

The nucleotide sequence files available below are those used to produce the plasmid vector, viral and bacteriophage maps contained in New England Biolabs Catalog as well as the tables containing the locations of sites. Maps and location of sites are PDF files. Sequence files are in FASTA or/and GenBank format. Detailed frequencies with which restriction endonuclease sites occur in commonly used DNA molecules can be found on Frequencies of Restriction Sites.

DNASU is a central repository for plasmid clones and collections that may also be helpful.

Vectors Vector Set Nucleotide Sequences Genbank Link Length (bp) Maps (pdf) Sites (pdf)
Adenovirus-2     GenBank 35937 Map Site
DHFR Control Template PURExpress  Fasta GenBank 2727    
FLuc Control Plasmid T7 High Yield
 Fasta GenBank 3925

Lambda    Fasta GenBank 48502 Map Site
Lambda_gt11(SacI-KpnI)    Fasta GenBank 1988 Map  
LITMUS-U USER  Fasta GenBank 2715    
LITMUS28 LITMUS  Fasta GenBank 2823    
LITMUS28i LITMUS  Fasta GenBank 2823 Map Site
LITMUS28i-Mal LITMUS  Fasta GenBank 3593    
LITMUS29 LITMUS  Fasta GenBank 2820    
LITMUS38 LITMUS  Fasta GenBank 2814    
LITMUS38i LITMUS  Fasta GenBank 2814 Map Site
LITMUS39 LITMUS  Fasta GenBank 2817    
M13KE PhD  Fasta GenBank 7222 Map Site
M13KO7    Fasta GenBank 8669    
M13mp/pUC         Map  
M13mp18    Fasta GenBank 7249 Map Site
pACP-ADRβ2 Cellular Imaging  Fasta GenBank 6824    
pACP-GPI Cellular Imaging  Fasta GenBank 5238    
pACP-tag(m) Cellular Imaging  Fasta GenBank 5088    
pACP-tag(m)-2 Cellular Imaging  Fasta GenBank 5543    
pACYC177    Fasta GenBank 3941 Map Site
pACYC184    Fasta GenBank 4245 Map Site
pBeloBAC11    Fasta GenBank 7507 Map Site
pBR322    Fasta GenBank 4361 Map Site
pCLIP-H2B Cellular Imaging  Fasta GenBank 6183    
pCLIP-NK1R Cellular Imaging  Fasta GenBank 7128    
pCLIP-tag(m) Cellular Imaging  Fasta GenBank 5805    
pCLIPf Cellular Imaging  Fasta GenBank 5862    
pCLIPf-Cox8A Cellular Imaging  Fasta GenBank 6077    
pCLIPf-H2B Cellular Imaging  Fasta GenBank 6249    
pCLIPf-NK1R Cellular Imaging  Fasta GenBank 7128    
pCLuc Mini-TK 2    Fasta GenBank 6131    
pCLuc-Basic 2 Luciferase  Fasta GenBank 6061    
pCMV-CLuc 2    Fasta GenBank 6905    
pCMV-GLuc Luciferase  Fasta GenBank 5764 Map  
pCMV-GLuc 2 Luciferase  Fasta GenBank 5764 Map  
pCYB1 IMPACT  Fasta GenBank 6846    
pCYB2 IMPACT  Fasta GenBank 6843    
pCYB3 IMPACT  Fasta GenBank 6846    
pCYB4 IMPACT  Fasta GenBank 6843    
pET11c pET  Fasta GenBank 5672 Map Site
pFOS1    Fasta GenBank 9738 Map Site
pGGA    Fasta GenBank 2174 Map  
pGGAselect    Fasta GenBank 2220 Map  
pGLuc Mini-TK Luciferase  Fasta GenBank 4990 Map  
pGLuc Mini-TK 2 Luciferase  Fasta GenBank 5028 Map  
pGLuc-Basic Luciferase  Fasta GenBank 4920 Map Site
pGLuc-Basic 2 Luciferase  Fasta GenBank 4958 Map
pGPS1.1 GPS  Fasta GenBank 4814 Map  
pGPS2.1 GPS  Fasta GenBank 4490 Map  
pGPS3 GPS  Fasta GenBank 4293 Map Site
pGPS4 GPS  Fasta GenBank 3899 Map  
pGPS5 GPS  Fasta GenBank 4223 Map  
pGT-N28 pGT  Fasta GenBank 4665    
pGT-N29 pGT  Fasta GenBank 4662    
pGT-N38 pGT  Fasta GenBank 4656    
pGT-N39 pGT  Fasta GenBank 4647    
phiX174     GenBank 5386 Map Site
pKLAC-malE Yeast  Fasta GenBank 10153    
pKLAC1 Yeast  Fasta GenBank 9091 Map Site
pKLAC1-GLuc Yeast  Fasta GenBank 9589 Map  
pKLAC2 Yeast  Fasta GenBank 9107 Map  
pKLMF-EK Yeast  Fasta GenBank 9991 Map  
pKLMF-FX Yeast  Fasta GenBank 9988 Map  
pKYB1 IMPACT  Fasta GenBank 8393    
pMAL-c pMAL  Fasta GenBank 6146    
pMAL-c2 pMAL  Fasta GenBank 6644    
pMAL-c2E pMAL  Fasta GenBank 6649    
pMAL-c2G pMAL  Fasta GenBank 6649    
pMAL-c2X pMAL  Fasta GenBank 6646    
pMAL-c4E pMAL  Fasta GenBank 6648    
pMAL-c4G pMAL  Fasta GenBank 6648    
pMAL-c4X pMAL  Fasta GenBank 6645    
pMAL-c5E pMAL  Fasta GenBank 5680    
pMAL-c5G pMAL  Fasta GenBank 5680    
pMAL-c5X pMAL  Fasta GenBank 5677    
pMAL  Fasta GenBank 5695    
pMAL-cRI pMAL  Fasta GenBank 6131    
pMAL-p pMAL  Fasta GenBank 6221    
pMAL-p2 pMAL  Fasta GenBank 6719    
pMAL-p2E pMAL  Fasta GenBank 6724    
pMAL-p2G pMAL  Fasta GenBank 6724    
pMAL-p2X pMAL  Fasta GenBank 6721 Map  
pMAL-p4E pMAL  Fasta GenBank 6723    
pMAL-p4G pMAL  Fasta GenBank 6723    
pMAL-p4X pMAL  Fasta GenBank 6720 Map Site
pMAL-p5E pMAL  Fasta GenBank 5755 Map  
pMAL-p5G pMAL  Fasta GenBank 5755 Map  
pMAL-p5X pMAL  Fasta GenBank 5752 Map  
pMAL-pIII PhD  Fasta GenBank 6706 Map Site
pMCP-GPI Cellular Imaging  Fasta GenBank 5238    
pMCP-tag(m) Cellular Imaging  Fasta GenBank 5088    
pMiniT PCR Cloning Kit  Fasta GenBank 2525 Map
pMiniT2.0 PCR Cloning Kit  Fasta GenBank 2588 Map
pMXB10 IMPACT  Fasta GenBank 7813 Map  
pMYB5 IMPACT  Fasta GenBank 8602    
pNEB193    Fasta GenBank 2713 Map Site
pNEB205a USER  Fasta GenBank 2710 Map  
pNEB206a USER  Fasta GenBank 2722 Map Site
pNEBR-R1 RheoSwitch  Fasta GenBank 10338    
pNEBR-R1Puro RheoSwitch  Fasta GenBank 9337    
pNEBR-X1 RheoSwitch  Fasta GenBank 4287 Map  
pNEBR-X1GLuc RheoSwitch  Fasta GenBank 4821 Map  
pNEBR-X1Hygro RheoSwitch  Fasta GenBank 6286 Map Site
pRS413 Yeast  Fasta GenBank 4970 Map Site
pRS414 Yeast  Fasta GenBank 4788 Map  
pRS415 Yeast  Fasta GenBank 6021 Map  
pRS416 Yeast  Fasta GenBank 4891 Map  
pSNAP-ADRB2 Cellular Imaging  Fasta GenBank 7122    
pSNAP-CaaX Control Plasmid Cellular Imaging  Fasta GenBank 5418 Map  
pSNAP-Cox8A Control Plasmid Cellular Imaging  Fasta GenBank 6015 Map  
pSNAP-H2B Cellular Imaging  Fasta GenBank 6183    
pSNAP-tag(m) Cellular Imaging  Fasta GenBank 5802 Map  
pSNAP-tag(m)2 Cellular Imaging  Fasta GenBank 5849    
pSNAP-tag(T7) Cellular Imaging  Fasta GenBank 3725    
pSNAP-tag(T7)2 Cellular Imaging  Fasta GenBank 6495    
pSNApf Cellular Imaging  Fasta GenBank 5849 Map  
pSNAPf-ADRβ2 Cellular Imaging  Fasta GenBank 7164    
pSNAPf-Cox8A Cellular Imaging  Fasta GenBank 6064    
pSNAPf-H2B Cellular Imaging  Fasta GenBank 6236    
pSV40-CLuc Luciferase  Fasta GenBank 5256    
pSV40-GLuc Luciferase  Fasta GenBank 4178 Map  
pTK-CLuc    Fasta GenBank 7000    
pTK-GLuc Luciferase  Fasta GenBank 5897 Map  
pTWIN-MBP1 IMPACT  Fasta GenBank 8515    
pTWIN1 IMPACT  Fasta GenBank 7375 Map Site
pTWIN2 IMPACT  Fasta GenBank 7192    
pTXB1 IMPACT  Fasta GenBank 6706 Map Site
pTXB3 IMPACT  Fasta GenBank 6906 Map  
pTYB1 IMPACT  Fasta GenBank 7477 Map Site
pTYB11 IMPACT  Fasta GenBank 7412 Map Site
pTYB12 IMPACT  Fasta GenBank 7415 Map  
pTYB2 IMPACT  Fasta GenBank 7474 Map  
pTYB21 IMPACT  Fasta GenBank 7514 Map  
pTYB22 IMPACT  Fasta GenBank 7511 Map  
pTYB3 IMPACT  Fasta GenBank 7477 Map  
pTYB4 IMPACT  Fasta GenBank 7474 Map  
pUC19    Fasta GenBank 2686 Map Site
PURExpress Univ Primer PURExpress  Fasta GenBank 85    
SV40     GenBank 5243 Map Site
T7     GenBank 39937 Map Site
YEp24 Yeast  Fasta GenBank 7769 Map Site
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