Interactive Tools

NEB Tools


  • NEBioCalculator
    Use this tool for your scientific calculations and conversions for DNA and RNA. Options include conversion of mass to moles, ligation amounts, conversion of OD to concentration, dilution and molarity. Additional features include sgRNA Template Oligo Design and qPCR library quantification.
  • ThermostableLigase_Calc_WebTool_NEB
    Thermostable Ligase Reaction Temperature Calculator
    This tool will estimate an optimal reaction temperature to minimize mismatch for thermostable ligation of two adjacent ssDNA probes annealed to a template.
  • ReadCoverageCalc_WebTool_NEB_noName
    Read Coverage Calculator
    This tool allows for easy calculation of values associated with read coverage in NGS protocols.
  • Tm Calculator
    Tm Calculator
    Use this tool when designing PCR reaction protocols to help determine the optimal annealing temperature for your amplicon. Simply input your DNA polymerase, primer concentration and your primer sequence and the Tm Calculator will guide you to successful reaction conditions.

Experimental Design

  • GlycanAnalyzer_WebTool_NEB
    Glycan Analyzer
    Use this tool to interpret Ultra or High Pressure liquid chromatography (UPLC/HPLC) N-glycan profiles following exoglycosidase digestions.
  • DNAsequenceMapWebToolNEB
    DNA Sequences and Maps Tool
    Use this tool to find the nucleotide sequence files for commonly used molecular biology tools, including plasmid, viral and bacteriophage vectors.
  • sgRNADesigner_WebTool_NEB_noName
    EnGen™ sgRNA Template Oligo Designer
    EnGen™ sgRNA Template Oligo Designer can be used to design target-specific DNA oligos for use with the EnGen™ sgRNA Synthesis Kit, S. pyogenes (NEB #E3322).
  • NEBNextCustomRNADepletion_WebTool_NEB_noName
    NEBNext Custom RNA Depletion Design Tool
    This tool designs probes to be used with the NEBNext RNA Depletion Core Reagent Set or to supplement an existing NEBNext Depletion Kit for the depletion of unwanted RNA species.
  • PCRFidelityEstimator_WebTool_NEB_noName
    PCR Fidelity Estimator
    Estimate the percentage of correct DNA copies (those without base substitution errors) per cycle of PCR for selected DNA polymerases.

Primer Design Tools

  • NEBuilder_WebTool_NEB
    NEBuilder Assembly Tool
    NEBuilder Assembly Tool can be used to design primers for your Gibson Assembly reaction, based on the entered fragment sequences and the polymerase being used for amplification.
  • NEBGoldenGate_WebTool_NEB
    NEB Golden Gate Assembly Tool
    Use this tool to assist with in silico DNA construct design for Golden Gate DNA assembly. It enables the accurate design of primers with appropriate type IIS restriction sites and overlaps, quick import of sequences in many formats and export of the final assembly, primers and settings.
  • LAMPPrimerDesigner_WebTool_NEB
    NEB LAMP Primer Design Tool
    NEB LAMP Primer Design Tool can be used to design primers for your Loop-mediated Isothermal Amplification. Fixed primers can be specified for the design of LAMP primers, and subsequent Loop primers are then designed based on LAMP primer selection.
  • NEBaseChanger_WebTool_NEB
    NEBaseChanger can be used to design primers specific to the mutagenesis experiment you are performing using the Q5 Site-Directed Mutagenesis Kit. This tool will also calculate a recommended custom annealing temperature based on the sequence of the primers by taking into account any mismatches.

Product Selection

  • Cross Ref
    Competitor Cross-Reference Tools
    Use this tool to select another company's product and find out which NEB product is compatible. Choose either the competitor’s product name or catalog number from the available selections, and this tool will identify the recommended NEB product.
  • NEBcloner
    Use this tool to find the right products and protocols for each step (digestion, end modification, ligation and transformation) of your next traditional cloning experiment. Also, find other relevant tools and resources to enable protocol optimization.
  • NEBNext Selector
    NEBNext Selector
    Use this tool to guide you through selection of NEBNext reagents for next generation sequencing sample preparation.
  • PCR Selector
    PCR Selector
    Use this tool to help select the right DNA polymerase for your PCR setup. Whether your amplicon is long, complex, GC-rich or present in a single copy, the PCR selection tool will identify the perfect DNA polymerase for your reaction.
  • ExoSelector_WebTool_NEB
    Exo Selector
    Use this tool to simplify the process of selecting the appropriate exonucleases for use in your nucleic acid digestion workflows. The tool guides you to product recommendations based on your answers to a few simple questions.

Restriction Enzyme Tools

  • Double Digest Finder
    Double Digest Finder
    Use this tool to guide your reaction buffer selection when setting up double-digests, a common timesaving procedure. Choosing the right buffers will help you to avoid star activity and loss of product.
  • Enzyme Finder
    Enzyme Finder
    Use this tool to select restriction enzymes by name, sequence, overhang or type. Enter your sequence using single letter code nomenclature, and Enzyme Finder will identify the right enzyme for the job.
  • NEB Cutter®
    NEBcutter V2.0
    Use this tool to identify the restriction sites within your DNA sequence. Choose between Type II and commercially available Type III restriction enzymes to digest your DNA. NEBcutter V2.0 will indicate cut frequency and methylation state sensitivity.