NEBNext End Repair Module Protocol (E6050)

Introduction

Starting Material: 1-5 μg of DNA Fragmented to 100-1000 bp in ≤ 85 μl

Protocol

1. Mix the following components in a sterile microfuge tube:
   
   

   Fragmented DNA	variable
   NEBNext End Repair Reaction Buffer (10X)	10 μl
   NEBNext End Repair Enzyme Mix	5 μl
   Sterile H2O for a final volume of 100 μl	variable
   Total volume	100 μl

   
   
   
2. Incubate in a thermocycler for 30 minutes at 20°C with heated lid set to 30°C (or off).
   
   
3. Purify DNA Sample using AMPure XP or SPRIselect beads.