Quickly and easily purify up to 100 µg of high quality total RNA from multiple sample types – all with one kit!
Check out our Technical Note containing comprehensive insights into measuring and analyzing nucleic acids.
The Monarch Total RNA Miniprep Kit is a comprehensive solution for sample preservation, cell lysis, gDNA removal, and purification of total RNA from a wide variety of biological samples, including cultured cells, blood, and mammalian tissues. Additionally, tough-to-lyse samples, such as bacteria, yeast, and plant, can be processed with additional steps that enhance lysis. Cleanup of enzymatic reactions or purification of RNA from TRIzol® -extracted samples is also possible using this kit. Purified RNA has high quality metrics, including A260/280 and A260/230 ratios >1.8, high RIN scores, and minimal residual gDNA. Captured RNA ranges in size from full-length rRNAs down to intact miRNAs. Additionally, differential binding conditions allow selective capture or exclusion of the sub-200 nucleotide RNA pool that includes miRNA, 5S rRNA, and tRNA. Purified RNA is suitable for downstream applications, such as RT-qPCR, cDNA synthesis, RNA-seq, Northern blot analysis, etc.
|Binding Capacity:||100 µg RNA|
|RNA Size:||≥ 20 nt|
|Purity:||A260/280 usually ≥ 1.8, A260/230 usually ≥ 1.8|
|Tissue Input:||up to 20 mg for high yield tissues|
|up to 50 mg for low yield tissues|
|Cultured Mammalian Cells and Blood Cells (PBMC's, WBC's) Input:||up to 1 x 107|
|Mammalian Whole Blood Input:||up to 3 ml|
|Bacteria Input:||up to 1 x 109|
|Yeast Input:||up to 5 x 107|
|Plant Input:||up to 100 mg|
|Elution Volume:||30 - 100 μl|
|Yield:||varies depending on sample type|
|Compatible downstream applications:||RNA Library prep for NGS, RT-PCR, RT-qPCR, Northern blots|
Validated Sample Types:
Additional Data Figures:
The following reagents are supplied with this product:
|NEB #||Component Name||Component||Stored at (°C)||Amount||Concentration|
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at [email protected] or 1-800-632-5227 x 8.
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.