Hi-T7® RNA Polymerase
NEBU recombinant engineered 50

Hi-T7 RNA Polymerase is designed for in vitro transcription of RNA at higher temperatures.

  • Supplied with 10X Hi-T7 RNA Polymerase Reaction Buffer formulated for optimal performance
  • Active from 37-56°C
  • For standard in vitro transcription at 37°C use T7 RNA Polymerase (NEB# M0251)
  • Increased co-transcriptional capping efficiency with cap analogs
  • Decreased immunogenicity from RNA synthesized at higher temperature due to reduced dsRNA by-product formation
  • High concentration format also available (NEB #M0470)
  • Getting ready to scale up RNA synthesis? Download our new technical note “Scaling of High-Yield In vitro Transcription Reactions for Linear Increase of RNA Production” for a generalized set of recommendations for synthesizing high yields of RNA.
Catalog Number Concentration Size List Price Your Price Quantity
M0658S 50,000 units/ml 5,000 units $89.00
Please enter a quantity for at least one size
Loading Spinner