EnGen® Sau Cas9
recombinant neb31 37 65 Heat

We are excited to announce that we are in the process of switching all reaction buffers to be BSA-free. Beginning April 2021, we will be gradually transitioning to buffers containing Recombinant Albumin (rAlbumin) for restriction enzymes and some DNA modifying enzymes. All information on the website has been updated to reflect this change. Find more details at www.neb.com/BSA-free.

EnGen® Sau Cas9 is a programmable DNA endonuclease guided by a single guide RNA (gRNA).  Targeting requires gRNA complementarity to the target site as well as a 5′NNGRRT protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence.  Cleavage by EnGen® Sau Cas9 occurs ~3 bases upstream of the PAM and leaves blunt ends.

  • Longer 5′ NNGRRT 3′ PAM sequence allows for higher specificity
  • Two nuclear localization signals for improved transport into the nucleus
  • High concentration liquid format option can be used for microinjection, electroporation and lipofection
  • Compatible with the EnGen® Mutation Detection Kit
  • Need guide RNA? NEB recommends the Horizon™ CRISPR Design Tool for designing and ordering synthetic guide RNA for EnGen Sau Cas9
Catalog # Concentration Size List Price Your Price Quantity
M0654S 1 µM 70 pmol $108.00
M0654T 20 µM 400 pmol $270.00
Please enter a quantity for at least one size
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