Home PCR, qPCR & Amplification Technologies Products Bst DNA Polymerase, Full Length

Bst DNA Polymerase, Full Length
NEBU cloned at NEB recombinant 65 80 Heat

The L size of this product has been discontinued.  The S size will still be available.

Optimal performance in a number of applications

  • Isothermal DNA amplification
  • Primer extension
  • Utilizes ThermoPol® Reaction Buffer for optimum reaction conditions

Blog_ContextualAd
Catalog # Concentration Size List Price Your Price Quantity
M0328S 5,000 units/ml 500 units $81.00
Please enter a quantity for at least one size
  • Bst DNA Polymerase, Full Length is the full length polymerase from Bacillus stearothermophilus. It has 5´ → 3´ polymerase and double-strand specific 5´ → 3´ exonuclease activity, but lacks 3´ → 5´ exonuclease activity (1).

    Product Source

    An E. coli strain that contains the gene from Bacillus stearothermophilus. (H. Kong)
    This product is related to the following categories:
    Isothermal Amplification & Strand Displacement Products
    This product can be used in the following applications:
    Isothermal Amplification

    • Reagents Supplied

      The following reagents are supplied with this product:

      NEB # Component Name Component # Stored at (°C) Amount Concentration

    • Properties & Usage

      Unit Definition

      One unit is defined as the amount of enzyme that will incorporate 10 nmol of dNTP into acid insoluble material in 30 minutes at 65°C.

      Reaction Conditions

      1X ThermoPol® Reaction Buffer
      Incubate at 65°C

      1X ThermoPol® Reaction Buffer
      20 mM Tris-HCl
      10 mM (NH4)2SO4
      10 mM KCl
      2 mM MgSO4
      0.1% Triton® X-100
      (pH 8.8 @ 25°C)

      Storage Buffer

      10 mM Tris-HCl
      50 mM KCl
      1 mM DTT
      0.1 mM EDTA
      50% Glycerol
      0.1% Triton® X-100
      pH 7.1 @ 25°C

      Heat Inactivation

      80°C for 20 minutes

      5' - 3' Exonuclease

      Yes

      3' - 5' Exonuclease

      No

      Strand Displacement

      No

      Unit Assay Conditions

      50 mM KCl, 20 mM Tris-HCl (pH 8.8), 10 mM MgCl2, 30 nM M13mp18 SS DNA, 70 nM M13 sequencing primer (–47) 24-mer, 200 µM dATP, 200 µM dCTP, 200 µM dGTP, 100 µM dTTP including [3H]-dTTP and 100 µg/ml BSA.

    • Related Products

      Companion Products

      Materials Sold Separately

    • Product Notes

      1. Bst DNA Polymerase does not exhibit 3´→ 5´ exonuclease activity.
      2. Reaction temperatures above 70°C are not recommended.
      3. Cannot be used for thermal cycle sequencing or PCR.

    • References

      1. Aliotta, J.M. et al. (1996). Genetic Analysis. 12, 185-195.
      2. Mead, D.A. et al. (1991). BioTechniques. 11, 76-87.

  • Tools & Resources

  • FAQs & Troubleshooting

  • Citations & Technical Literature

  • Quality, Safety & Legal

    • Quality Control Assays

      Quality Control tests are performed on each new lot of NEB product to meet the specifications designated for it. Specifications and individual lot data from the tests that are performed for this particular product can be found and downloaded on the Product Specification Sheet, Certificate of Analysis, data card or product manual. Further information regarding NEB product quality can be found here.

    • Specifications & Change Notifications

      Effective March 15, 2022, RNase method incubation time has changed to 4 hours.
      The Specification sheet is a document that includes the storage temperature, shelf life and the specifications designated for the product. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]

    • Certificate of Analysis

      The Certificate of Analysis (COA) is a signed document that includes the storage temperature, expiration date and quality controls for an individual lot. The following file naming structure is used to name these document files: [Product Number]_[Size]_[Version]_[Lot Number]

    • Safety Data Sheets

      The following is a list of Safety Data Sheet (SDS) that apply to this product to help you use it safely.

    • Legal and Disclaimers

      Products and content are covered by one or more patents, trademarks and/or copyrights owned or controlled by New England Biolabs, Inc (NEB). The use of trademark symbols does not necessarily indicate that the name is trademarked in the country where it is being read; it indicates where the content was originally developed. The use of this product may require the buyer to obtain additional third-party intellectual property rights for certain applications. For more information, please email busdev@neb.com.

      This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

      New England Biolabs (NEB) is committed to practicing ethical science – we believe it is our job as researchers to ask the important questions that when answered will help preserve our quality of life and the world that we live in. However, this research should always be done in safe and ethical manner. Learn more.

      Trademarks

      NEW ENGLAND BIOLABS® and THERMOPOL® are registered trademarks of New England Biolabs, Inc.
      TRITON® is a registered trademark of Union Carbide Corporation.

Other Products You May Be Interested In

Ineligible item added to cart

Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at freezers@neb.com or 1-800-632-5227 x 8.

Continue

Choose your country

North America

Europe

Asia-Pacific

If you don't see your country above, please visit our international site

animated golden butterfly flying around

Find a Golden Butterfly for a Chance to Win!

Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.

Learn More
Remind me next time

Session Expired

You have been idle for more than 20 minutes, for your security you have been logged out. Please sign back in to continue your session.

Institution Changed

Your profile has been mapped to an Institution, please sign back for your profile updates to be completed.

Sign in to your NEB account

To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.