Featured Video
-
Product Information
T7 DNA Ligase is an ATP-dependent ds DNA ligase from bacteriophage T7. It will catalyze the formation of a phosphodiester bond between adjacent 5´ phosphate and 3´ hydroxyl groups of duplex DNA. Cohesive end ligation and nick sealing can be efficiently catalyzed by T7 DNA Ligase (1,2). However, unlike T4 and T3 DNA Ligases, blunt end ligation is not efficiently catalyzed by T7 DNA Ligase. Addition of high concentrations of PEG 6000 [≥ 20% (w/v)] to the reaction can force T7 DNA Ligase to have measurable activity. However, under typical reaction conditions blunt-end DNA ligation does not occur in the presence of T7 DNA Ligase, making it a good choice for applications in which blunt and cohesive ends of DNA are present but only the cohesive ends are to be joined.
T7 DNA Ligase does not ligate blunt-end DNA fragments. Ligation reactions containing blunt fragments (ΦX174 DNA-HaeIII Digest (NEB #N3026 ) and sticky-end fragments (λ DNA-HindIII Digest (NEB #N3012 ) were set up with 200 ng of each substrate and 1 μl of each ligase, and incubated for 30 minutes at 25°C in their corresponding reaction buffers. Reactions were immediately stopped with 6X loading dye and resolved by electrophoresis on a 1% agarose gel and stained with ethidium bromide. Product Source
An E. coli strain containing a recombinant gene encoding T7 DNA Ligase.- This product is related to the following categories:
- DNA Ligases Products
- This product can be used in the following applications:
- Cloning Ligation,
- NEBridge® Golden Gate Assembly
-
Protocols, Manuals & Usage
-
Tools & Resources
-
FAQs & Troubleshooting
-
Citations & Technical Literature
-
Product Citation Tool
Other Products You May Be Interested In
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at freezers@neb.com or 1-800-632-5227 x 8.
Powered by Bioz
Choose your country
North America
Europe
Asia-Pacific
Find a Golden Butterfly for a Chance to Win!
Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.