The NEBExpress® Cell-free E. coli Protein Synthesis System is an extract-based transcription/translation system derived from E. coli cells that have been engineered for high in vitro synthesis performance. It is designed to synthesize proteins ranging in size from 17-230 kDa in just 2-4 hours under the control of T7 RNA Polymerase.
The following reagents are supplied with this product:
|Store at (°C)||Concentration|
|NEBExpress™ S30 Synthesis Extract||-80|
|Protein Synthesis Buffer (2X)||-80||2 X|
|RNase Inhibitor, Murine||-20||40,000 units/ml|
|T7 RNA Polymerase||-20|
|Control DHFR-His Template||-80||125 ng/µl|
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