Need a custom/large volume order? Contact us
-
Product Information
Chemically competent E. coli cells suitable for high efficiency transformation in a wide variety of applications.
Highlights
- DH10B™ derivative
- Efficient transformation of methylated DNA derived from eukaryotic sources or unmethylated DNA derived from PCR, cDNA and many other sources [mcrAΔ(mrr-hsdRMS-mcrBC)]
- Activity of nonspecific endonuclease I (endA1) eliminated for highest quality plasmid preparations
- Resistance to phage T1 (fhuA)
- Suitable for blue/white screening without IPTG by a-complementation of the b-galactosidase gene
- Reduced recombination of cloned DNA (recA1)
- Clone large plasmids and BACs
- Free of animal products
- K12 Strain
Transformation Efficiency
For C3019I and C3019H: 1–3 x 109 cfu/µg pUC19 DNA
For C3019P: 1–3 x 108 cfu/µg pUC19 DNAGenotype
Δ(ara-leu) 7697 araD139 fhuA ΔlacX74 galK16 galE15 e14- ϕ80dlacZΔM15 recA1 relA1 endA1 nupG rpsL (StrR) rph spoT1 Δ(mrr-hsdRMS-mcrBC)- This product is related to the following categories:
- Cloning Competent Cell Strains Products
- This product can be used in the following applications:
- USER® Cloning,
- Applications of USER® and Thermolabile USER II Enzymes,
- Site Directed Mutagenesis,
- Site Directed Mutagenesis,
- Transformation, High-throughput cloning and automation solutions
-
Protocols, Manuals & Usage
-
Tools & Resources
-
FAQs & Troubleshooting
-
Citations & Technical Literature
-
Product Citation Tool
-
Featured Videos
-
Tips for Successful Transformations with NEB® Competent Cells
-
How to Perform a Transformation with NEB® Competent Cells
-
The Mechanism of Transformation with Competent Cells
-
Overview of PCR Cloning
-
Traditional Cloning Workflow
-
NEBUILDER HIFI DNA ASSEMBLY® Removal of 3´ end Mismatches
-
Fast transformation plating with glass beads
Ineligible item added to cart
Based on your Freezer Program type, you are trying to add a product to your cart that is either not allowed or not allowed with the existing contents of your cart. Please review and update your order accordingly If you have any questions, please contact Customer Service at freezers@neb.com or 1-800-632-5227 x 8.
Powered by Bioz
Choose your country
North America
Europe
Asia-Pacific
Find a Golden Butterfly for a Chance to Win!
Help us celebrate our 50th anniversary! We have hidden 1,000 golden butterflies and are waiting for you to find them. They can be anywhere that you find NEB! Beginning April 15th, be sure to visit our website and tables at tradeshows and events you are attending. Visit our social media channels frequently for tips on where we have hidden the butterflies – and once you find one, either click or scan the code to be eligible for a 50th anniversary prize pack, as well as a grand prize trip to NEB headquarters in Ipswich, MA!.
To save your cart and view previous orders, sign in to your NEB account. Adding products to your cart without being signed in will result in a loss of your cart when you do sign in or leave the site.