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Chemically competent E. coli cells derived from BL21(DE3). Poly-histidine tagged recombinant proteins that are isolated by immobilized metal affinity chromatography (IMAC) are often contaminated with significant amounts of endogenous E. coli metal binding proteins. The protein expression strain NiCo21(DE3) has been engineered to minimize E. coli protein contamination of IMAC fractions: GlmS is mutated to eliminate binding to IMAC resins and three other proteins (SlyD, ArnA and Can) are tagged to enable rapid removal by chitin affinity chromatography. Highlights
- Superior alternative to BL21(DE3) for routine protein expression
- Improved purity of target proteins isolated by IMAC
- Identical growth characteristics as BL21(DE3)
- Deficient in proteases Lon and OmpT
- Resistant to phage T1 (fhuA2)
- Free of animal products
Transformation Efficiency
1–5 x 107 cfu/µg pUC19Genotype
can::CBD fhuA2 [lon] ompT gal (λ DE3) [dcm] arnA::CBD slyD::CBD glmS6Ala ∆hsdS λ DE3 = λ sBamHIo ∆EcoRI-B int::(lacI::PlacUV5::T7 gene1) i21 ∆nin5- This product is related to the following categories:
- T7 Expression,
- Nickel Purification (His-tag),
- Affinity Purification Products,
- Protein Purification Products,
- E. coli Protein Expression Strains Products,
- Protein Expression Products, E. coli Expression Strains Products
- This product can be used in the following applications:
- His-tagged Protein Expression & Purification,
- Affinity Purification & Expression Tags,
- Protein Purification,
- T7 Expression,
- Peptide Ligation,
- Protein Expression in E. Coli, Protein Expression
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