Home FAQs Can the Unstained Protein Standard, Broad Range be used as a precise standard for determining the exact molecular weight of a protein of interest?

FAQ: Can the Unstained Protein Standard, Broad Range be used as a precise standard for determining the exact molecular weight of a protein of interest?

Molecular weight determination by SDS-PAGE is a dependable method. However, an unknown protein’s molecular weight should be obtained by mass spectrometry if a precise molecular weight determination is required. Mass spectrometry has a higher degree of accuracy because each amino acid of a protein is analyzed.