FAQ: Why are my sequence reads less than 600 bases even when the electropherogram looks great?

This could be the result of submitting too much sample for sequencing and overloading the BigDye™ terminator chemistry. Make sure the submitted sample is between 15-200 fmol in a 3 µl volume. This corresponds to the following mass amounts:

Size of PCR amplicon ng of DNA (in 3 ul sample)
100 bp 1 - 12
500 bp 5 - 60
1000 bp 10 - 120
3000 bp 30 - 360
5000 bp 50 - 600