FAQ: What is the difference between the NEBNext Ultra II Directional RNA Library Prep Kit (NEB #E7760) and the original NEBNext Ultra Directional RNA Library Prep Kit (NEB #E7420)?

Each step in the workflow for the NEBNext Ultra II Directional RNA Library Prep Kit has been optimized, including the incorporation of several new reagents. This enables the use of 10 to 20-fold lower input amounts and fewer PCR cycles compared to the original kit, and results in library yields that are substantially higher, without compromising library quality. Strand Specificity Reagent is also now included.