Home FAQs I’m doing a moderate (4–5 insert) assembly but don’t have access to a thermal cycler. Can I use the simpler protocol using the 1 hr 37°C incubation?

FAQ: I’m doing a moderate (4–5 insert) assembly but don’t have access to a thermal cycler. Can I use the simpler protocol using the 1 hr 37°C incubation?

Yes, but anticipate your assembly efficiency will decrease 4-fold; plate a larger amount of outgrowth to compensate.