FAQ: How much RNA template should I use in my RT-qPCR reaction?

The Luna® Universal One-Step RT-qPCR Kit works well with purified RNA templates including total RNA, mRNA, and in vitro transcript samples.

  Amount in 20 μl reaction 
total RNA   ≤ 1 μg
poly(A)-RNA  ≤ 0.1 μg
in vitro transcripts ≤ 10⌃9 copies