HomeFAQsIs it necessary to phosphatase treat my sgRNAs?
FAQ: Is it necessary to phosphatase treat my sgRNAs?
RNAs transcribed with a bacteriophage polymerase will possess 5’ triphosphates. Phosphatase treatment will remove these triphosphates leaving a 5’ OH. If treating with phosphatase we recommend treatment following DNaseI treatment. The enzyme can not be inactivated by heat, therefore phenol:chloroform extraction is the preferred method for inactivating the enzyme.