Home FAQs The recommended volume for a Blunt/TA Ligase Master Mix reaction is 10ul. I like to set-up my ligation reactions in a 20 ul volume. Can I scale-up the reaction?

FAQ: The recommended volume for a Blunt/TA Ligase Master Mix reaction is 10ul. I like to set-up my ligation reactions in a 20 ul volume. Can I scale-up the reaction?

Yes. As long as the concentration of the Blunt/TA Ligase Master Mix in the reaction is 1X (or 50% of the total reaction volume), you can scale up the ligation reaction.