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Protein Expression Products

New England Biolabs is committed to providing advanced tools for protein expression. As a leading supplier for reagents in the biotechnology field, NEB understands the importance of convenient and easy-to-use systems for high level expression and purification of proteins. We invite you to review our growing range of expression systems, including our new line of NEBExpress^® products, resulting from our 40+ years of experience in cloning, over-expression and purification. We use these solutions in our own research and manufacturing processes, and know that quality and performance are critical – all of our products are stringently tested so that you can be sure they will work optimally for your solution, just as we rely on them to work in ours.

Choose Product:

Protein Expression
Cell-Free Protein Expression
Protein Expression in Yeast
Expression of Difficult Proteins
Protein Expression in E. Coli

Protein Expression

Order BL21 Competent E. coli

Order BL21(DE3) Competent E. coli

Order E. coli Ribosome

Order Factor Xa Protease

Order IMPACT™ Kit

Order K. lactis GG799 Competent Cells

Order K. lactis Protein Expression Kit

Order Lemo21(DE3) Competent E. coli

Order NEBExpress® Cell-free E. coli Protein Synthesis System

Order NEBExpress® Competent E. coli (High Efficiency)

Order NEBExpress® GamS Nuclease Inhibitor

Order NEBExpress® I^q Competent E. coli (High Efficiency)

Order NEBExpress^® MBP Fusion and Purification System

Order NiCo21(DE3) Competent E. coli

Order pMAL-c6T Vector

Order pMAL™ Protein Fusion and Purification System

Order pMAL™-c5X Vector

Order pMAL™-p5X Vector

Order pTXB1 Vector

Order pTYB21 Vector

Order PURExpress® Disulfide Bond Enhancer

Order PURExpress® In Vitro Protein Synthesis Kit

Order PURExpress® Δ (aa, tRNA) Kit

Order PURExpress® Δ RF123 Kit

Order PURExpress® Δ Ribosome Kit

Order RNase Inhibitor, Murine

Order SHuffle® Express Competent E. coli

Order SHuffle® T7 Competent E. coli

Order SHuffle® T7 Express Competent E. coli

Order SHuffle® T7 Express lysY Competent E. coli

Order T7 Express Competent E. coli (High Efficiency)

Order T7 Express lysY Competent E. coli (High Efficiency)

Order T7 Express lysY/I^q Competent E. coli (High Efficiency)

Order TEV Protease

Order Yeast Carbon Base Medium Powder

Cell-Free Protein Expression

Order E. coli Ribosome

Order NEBExpress® Cell-free E. coli Protein Synthesis System

Order NEBExpress® GamS Nuclease Inhibitor

Order PURExpress® Disulfide Bond Enhancer

Order PURExpress® In Vitro Protein Synthesis Kit

Order PURExpress® Δ (aa, tRNA) Kit

Order PURExpress® Δ RF123 Kit

Order PURExpress® Δ Ribosome Kit

Order RNase Inhibitor, Murine

Protein Expression in Yeast

Order K. lactis GG799 Competent Cells

Order K. lactis Protein Expression Kit

Order Yeast Carbon Base Medium Powder

Expression of Difficult Proteins

Order Factor Xa Protease

Order Lemo21(DE3) Competent E. coli

Order NEBExpress^® MBP Fusion and Purification System

Order pMAL-c6T Vector

Order pMAL™ Protein Fusion and Purification System

Order PURExpress® Disulfide Bond Enhancer

Order PURExpress® In Vitro Protein Synthesis Kit

Order SHuffle® Express Competent E. coli

Order SHuffle® T7 Competent E. coli

Order SHuffle® T7 Express Competent E. coli

Order SHuffle® T7 Express lysY Competent E. coli

Order T7 Express lysY Competent E. coli (High Efficiency)

Order T7 Express lysY/I^q Competent E. coli (High Efficiency)

Order TEV Protease

Protein Expression in E. Coli

Order BL21 Competent E. coli

Order BL21(DE3) Competent E. coli

Order IMPACT™ Kit

Order Lemo21(DE3) Competent E. coli

Order NEBExpress® Competent E. coli (High Efficiency)

Order NEBExpress® I^q Competent E. coli (High Efficiency)

Order NEBExpress^® MBP Fusion and Purification System

Order NiCo21(DE3) Competent E. coli

Order pMAL-c6T Vector

Order pMAL™ Protein Fusion and Purification System

Order pMAL™-c5X Vector

Order pMAL™-p5X Vector

Order pTXB1 Vector

Order pTYB21 Vector

Order SHuffle® Express Competent E. coli

Order SHuffle® T7 Competent E. coli

Order SHuffle® T7 Express Competent E. coli

Order SHuffle® T7 Express lysY Competent E. coli

Order T7 Express Competent E. coli (High Efficiency)

Order T7 Express lysY Competent E. coli (High Efficiency)

Order T7 Express lysY/I^q Competent E. coli (High Efficiency)

Cell-Free Protein Expression

Order E. coli Ribosome

Order NEBExpress® Cell-free E. coli Protein Synthesis System

Order NEBExpress® GamS Nuclease Inhibitor

Order PURExpress® Disulfide Bond Enhancer

Order PURExpress® In Vitro Protein Synthesis Kit

Order PURExpress® Δ (aa, tRNA) Kit

Order PURExpress® Δ RF123 Kit

Order PURExpress® Δ Ribosome Kit

Order RNase Inhibitor, Murine

NEBExpress^® Cell-free E. coli Protein Synthesis System

Order NEBExpress® Cell-free E. coli Protein Synthesis System

Order NEBExpress® GamS Nuclease Inhibitor

Order PURExpress® Disulfide Bond Enhancer

Order RNase Inhibitor, Murine

PURExpress

Order E. coli Ribosome

Order PURExpress® Disulfide Bond Enhancer

Order PURExpress® In Vitro Protein Synthesis Kit

Order PURExpress® Δ (aa, tRNA) Kit

Order PURExpress® Δ RF123 Kit

Order PURExpress® Δ Ribosome Kit

Order RNase Inhibitor, Murine

Protein Expression in Yeast

Order K. lactis GG799 Competent Cells

Order K. lactis Protein Expression Kit

Order Yeast Carbon Base Medium Powder

Expression of Difficult Proteins

Order Lemo21(DE3) Competent E. coli

Order NEBExpress^® MBP Fusion and Purification System

Order pMAL-c6T Vector

Order PURExpress® Disulfide Bond Enhancer

Order PURExpress® In Vitro Protein Synthesis Kit

Order SHuffle® Express Competent E. coli

Order SHuffle® T7 Competent E. coli

Order SHuffle® T7 Express Competent E. coli

Order SHuffle® T7 Express lysY Competent E. coli

Order T7 Express lysY Competent E. coli (High Efficiency)

Order T7 Express lysY/I^q Competent E. coli (High Efficiency)

Disulfide-bonded Protein Expression

Order PURExpress® Disulfide Bond Enhancer

Order PURExpress® In Vitro Protein Synthesis Kit

Order SHuffle® Express Competent E. coli

Order SHuffle® T7 Competent E. coli

Order SHuffle® T7 Express Competent E. coli

Order SHuffle® T7 Express lysY Competent E. coli

Membrane Protein Expression

Order Lemo21(DE3) Competent E. coli

Order T7 Express lysY/I^q Competent E. coli (High Efficiency)

Toxic Protein Expression

Order Lemo21(DE3) Competent E. coli

Order PURExpress® In Vitro Protein Synthesis Kit

Order T7 Express lysY Competent E. coli (High Efficiency)

Order T7 Express lysY/I^q Competent E. coli (High Efficiency)

Target Protein Insolubility

Order Factor Xa Protease

Order NEBExpress^® MBP Fusion and Purification System

Order pMAL-c6T Vector

Order pMAL™ Protein Fusion and Purification System

Order TEV Protease

Protein Expression in E. Coli

Order BL21 Competent E. coli

Order BL21(DE3) Competent E. coli

Order IMPACT™ Kit

Order Lemo21(DE3) Competent E. coli

Order NEBExpress® Competent E. coli (High Efficiency)

Order NEBExpress® I^q Competent E. coli (High Efficiency)

Order NEBExpress^® MBP Fusion and Purification System

Order NiCo21(DE3) Competent E. coli

Order pMAL-c6T Vector

Order pMAL™ Protein Fusion and Purification System

Order pMAL™-c5X Vector

Order pMAL™-p5X Vector

Order pTXB1 Vector

Order pTYB21 Vector

Order SHuffle® Express Competent E. coli

Order SHuffle® T7 Competent E. coli

Order SHuffle® T7 Express Competent E. coli

Order SHuffle® T7 Express lysY Competent E. coli

Order T7 Express Competent E. coli (High Efficiency)

Order T7 Express lysY Competent E. coli (High Efficiency)

Order T7 Express lysY/I^q Competent E. coli (High Efficiency)

T7 Expression

Order BL21(DE3) Competent E. coli

Order Lemo21(DE3) Competent E. coli

Order NiCo21(DE3) Competent E. coli

Order SHuffle® Express Competent E. coli

Order SHuffle® T7 Competent E. coli

Order SHuffle® T7 Express Competent E. coli

Order SHuffle® T7 Express lysY Competent E. coli

Order T7 Express Competent E. coli (High Efficiency)

Order T7 Express lysY Competent E. coli (High Efficiency)

Order T7 Express lysY/I^q Competent E. coli (High Efficiency)

Non-T7 Expression

Order BL21 Competent E. coli

Order NEBExpress® Competent E. coli (High Efficiency)

Order NEBExpress® I^q Competent E. coli (High Efficiency)

Order NEBExpress^® MBP Fusion and Purification System

Order pMAL-c6T Vector

Order pMAL™ Protein Fusion and Purification System

New Products

NEBExpress® Cell-free E. coli Protein Synthesis System
NEBExpress^® MBP Fusion and Purification System
NEBExpress® GamS Nuclease Inhibitor
pMAL-c6T Vector

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Choose Product:

Order BL21 Competent E. coli

Order BL21(DE3) Competent E. coli

Order E. coli Ribosome

Order Factor Xa Protease

Order IMPACT™ Kit

Order K. lactis GG799 Competent Cells

Order K. lactis Protein Expression Kit

Order Lemo21(DE3) Competent E. coli

Order NEBExpress® Cell-free E. coli Protein Synthesis System

Order NEBExpress® Competent E. coli (High Efficiency)

Order NEBExpress® GamS Nuclease Inhibitor

Order NEBExpress® Iq Competent E. coli (High Efficiency)

Order NEBExpress® MBP Fusion and Purification System

Order NiCo21(DE3) Competent E. coli

Order pMAL-c6T Vector

Order pMAL™ Protein Fusion and Purification System

Order pMAL™-c5X Vector

Order pMAL™-p5X Vector

Order pTXB1 Vector

Order pTYB21 Vector

Order PURExpress® Disulfide Bond Enhancer

Order PURExpress® In Vitro Protein Synthesis Kit

Order PURExpress® Δ (aa, tRNA) Kit

Order PURExpress® Δ RF123 Kit

Order PURExpress® Δ Ribosome Kit

Order RNase Inhibitor, Murine

Order SHuffle® Express Competent E. coli

Order SHuffle® T7 Competent E. coli

Order SHuffle® T7 Express Competent E. coli

Order SHuffle® T7 Express lysY Competent E. coli

Order T7 Express Competent E. coli (High Efficiency)

Order T7 Express lysY Competent E. coli (High Efficiency)

Order T7 Express lysY/Iq Competent E. coli (High Efficiency)

Order TEV Protease

Order Yeast Carbon Base Medium Powder

Cell-Free Protein Expression

Order E. coli Ribosome

Order NEBExpress® Cell-free E. coli Protein Synthesis System

Order NEBExpress® GamS Nuclease Inhibitor

Order PURExpress® Disulfide Bond Enhancer

Order PURExpress® In Vitro Protein Synthesis Kit

Order PURExpress® Δ (aa, tRNA) Kit

Order PURExpress® Δ RF123 Kit

Order PURExpress® Δ Ribosome Kit

Order RNase Inhibitor, Murine

Protein Expression in Yeast

Order K. lactis GG799 Competent Cells

Order K. lactis Protein Expression Kit

Order Yeast Carbon Base Medium Powder

Expression of Difficult Proteins

Order Factor Xa Protease

Order Lemo21(DE3) Competent E. coli

Order NEBExpress® MBP Fusion and Purification System

Order pMAL-c6T Vector

Order pMAL™ Protein Fusion and Purification System

Order PURExpress® Disulfide Bond Enhancer

Order PURExpress® In Vitro Protein Synthesis Kit

Order SHuffle® Express Competent E. coli

Order SHuffle® T7 Competent E. coli

Order SHuffle® T7 Express Competent E. coli

Order SHuffle® T7 Express lysY Competent E. coli

Order T7 Express lysY Competent E. coli (High Efficiency)

Order T7 Express lysY/Iq Competent E. coli (High Efficiency)

Order TEV Protease

Protein Expression in E. Coli

Order BL21 Competent E. coli

Order BL21(DE3) Competent E. coli

Order IMPACT™ Kit

Order Lemo21(DE3) Competent E. coli

Order NEBExpress® Competent E. coli (High Efficiency)

Order NEBExpress® Iq Competent E. coli (High Efficiency)

Order NEBExpress® MBP Fusion and Purification System

Order NiCo21(DE3) Competent E. coli

Order pMAL-c6T Vector

Order pMAL™ Protein Fusion and Purification System

Order pMAL™-c5X Vector

Order pMAL™-p5X Vector

Order pTXB1 Vector

Order pTYB21 Vector

Order NEBExpress® I^q Competent E. coli (High Efficiency)

Order NEBExpress^® MBP Fusion and Purification System

Order T7 Express lysY/I^q Competent E. coli (High Efficiency)

Order NEBExpress^® MBP Fusion and Purification System

Order T7 Express lysY/I^q Competent E. coli (High Efficiency)

Order NEBExpress® I^q Competent E. coli (High Efficiency)

Order NEBExpress^® MBP Fusion and Purification System

Order T7 Express lysY/I^q Competent E. coli (High Efficiency)

NEBExpress^® Cell-free E. coli Protein Synthesis System

Order NEBExpress^® MBP Fusion and Purification System

Order T7 Express lysY/I^q Competent E. coli (High Efficiency)

Order T7 Express lysY/I^q Competent E. coli (High Efficiency)