
Glycobiology & Proteomics
Choose Type:
- What are glycosidases and their uses?
- Which proteins are glycosylated?
- Can glycosidases be used in combination for extensive digestion?
- Can I analyze protein glycosylation with only benchtop instrumentation and reagents?
- Do I need to deglycosylate my protein sample for proteomic analysis?
- How can I separate a deglycosylated protein from the glycosidase?
- Is it possible to predict whether a protein is N- or O-glycosylated?
- What are the advantages of enzymatic deglycosylation?
- What are the best techniques to analyze N- and O-glycans?
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Glycomics: A rapidly evolving field with a sweet future
Glycans are widely distributed in nature and have physical, chemical, and biological properties that make them important players in areas such as biofuels, food, materials science, biotechnology, and pharmaceuticals.
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N-Glycan Composition Profiling for Quality Testing of Biotherapeutics
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The Structure, Function and Importance of Carbohydrates
Read about the structure, function, and importance of Carbohydrates from biology experts at NEB.
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Using Glycosidases to Remove Trim or Modify Glycans on Therapeutic Protein
- Glycoproteomics Brochure
- Glycoproteomics Technical Guide
- Rapid PNGase F Trifold
- Glycan Analyzer
- Endoglycosidase Selection Chart
- Detailed Characterization of Several Glycosidase Enzymes
- Glycobiology Unit Conversion Chart
- Improving Sample Workflow: Rapid PNGase F for Accurate Antibody Characterization (2015)
Feature Articles
Brochures
Web Tools
Selection Tools
Usage Guidelines
Posters
- Chuzel, L.O., Ganatra, M.B., Rapp, E., Henrissat, B., Taron, C.H. 2018. Functional metagenomics identifies an exosialidase with an inverting catalytic mechanism that defines a new glycoside hydrolase family (GH156) J. Biol Chem. 293(47), PubMedID: 30249617, DOI:
- Vainauskas, S., Kirk, C.H., Petralia, L., Guthrie, E.P., McLeod, E., Bielik, A., Luebbers, A., Foster, J.M., Hokke, C.H., Rudd, P.M., Shi, X., Taron, C.H 2018. A novel broad specificity fucosidase capable of core a1-6 fucose release from N-glycans labeled with urea-linked fluorescent dyes Sci Rep. , PubMedID: 29934601, DOI: 1038/s41598-018-27797-0
- O'Flaherty, Roisin, Harbison, Aoife M., Hanley, Philip J., Fadda, Elisa, Rudd, Pauline, Taron, Chris 2017. Aminoquinoline fluorescent labels obstruct efficient removal of N-glycan corex Journal of Proteome Research. 16, PubMedID: , DOI:
- Albrecht, S., Vainauskas, S., Stöckmann, H., McManus, C., Taron, C.H. and Rudd, P.M. 2016. Comprehensive Profiling of Glycosphingolipid Glycans Using a Novel Broad Specificity Endoglycoceramidase in a High-Throughput Workflow. . May 3;88(9), PubMedID: 27033327, DOI:
- Itahana Y, Han R, Barbier S, Lei Z, Rozen S, Itahana K 2014. The uric acid transporter SLC2A9 is a direct target gene of the tumor suppressor p53 contributing to antioxidant defense Oncogene. , PubMedID: 24858040, DOI: 10.1038/onc.2014.119
- Arakel EC, Brandenburg S, Uchida K, Zhang H, Lin YW, Kohl T, Schrul B, Sulkin MS, Efimov IR, Nichols CG, Lehnart SE, Schwappach B 2014. Tuning the electrical properties of the heart by differential trafficking of KATP ion channel complexes J Cell Sci. 127(Pt 9), PubMedID: 24569881, DOI: 10.1242/jcs.141440
- Botto L, Cunati D, Coco S, Sesana S, Bulbarelli A, Biasini E, Colombo L, Negro A, Chiesa R, Masserini M, Palestini P 2014. Role of lipid rafts and GM1 in the segregation and processing of prion protein PLoS One. 9(5), PubMedID: 24859148, DOI: 10.1371/journal.pone.0098344
- Zhao H, Blazanovic K, Choi Y, Bailey-Kellogg C, Griswold KE 2014. Gene and protein sequence optimization for high-level production of fully active and aglycosylated lysostaphin in Pichia pastoris Appl Environ Microbiol. 80(9), PubMedID: 24561590, DOI: 10.1128/AEM.03914-13
- Möykkynen T, Coleman SK, Semenov A, Keinänen K 2014. The N-terminal domain modulates α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor desensitization J Biol Chem. 289(19), PubMedID: 24652293, DOI: 10.1074/jbc.M113.526301
- Stech M, Quast RB, Sachse R, Schulze C, Wüstenhagen DA, Kubick S 2014. A continuous-exchange cell-free protein synthesis system based on extracts from cultured insect cells PLoS One. 9(5), PubMedID: 24804975, DOI: 10.1371/journal.pone.0096635
- Kwon HM, Lee KH, Han BW, Han MR, Kim DH, Kim DE 2014. An RNA aptamer that specifically binds to the glycosylated hemagglutinin of avian influenza virus and suppresses viral infection in cells PLoS One. 9(5), PubMedID: 24835440, DOI: 10.1371/journal.pone.0097574
- Wright CR, Brown EL, Della-Gatta PA, Ward AC, Lynch GS, Russell AP 2014. G-CSF does not influence C2C12 myogenesis despite receptor expression in healthy and dystrophic skeletal muscle Front Physiol. 5, PubMedID: 24822049, DOI: 10.3389/fphys.2014.00170
- Wicht O, Burkard C, de Haan CA, van Kuppeveld FJ, Rottier PJ, Bosch BJ 2014. Identification and Characterization of a Proteolytically Primed Form of the Murine Coronavirus Spike Proteins after Fusion with the Target Cell J Virol. 88(9), PubMedID: 24554652, DOI: 10.1128/JVI.03451-13
- Haller G, Li P, Esch C, Hsu S, Goate AM, Steinbach JH 2014. Functional characterization improves associations between rare non-synonymous variants in CHRNB4 and smoking behavior PLoS One. 9(5), PubMedID: 24804708, DOI: 10.1371/journal.pone.0096753
- Rosenbaum EE, Vasiljevic E, Brehm KS, Colley NJ 2014. Mutations in four glycosyl hydrolases reveal a highly coordinated pathway for rhodopsin biosynthesis and N-glycan trimming in Drosophila melanogaster PLoS Genet. 10(5), PubMedID: 24785692, DOI: 10.1371/journal.pgen.1004349
- Rosenbaek LL, Kortenoeven ML, Aroankins TS, Fenton RA 2014. Phosphorylation decreases ubiquitylation of the thiazide-sensitive cotransporter NCC and subsequent clathrin-mediated endocytosis J Biol Chem. 289(19), PubMedID: 24668812, DOI: 10.1074/jbc.M113.543710
- Velho, A.M., Jarvis, S.M. 2009. Topological studies of hSVCT1, the human sodium-dependent vitamin C transporter and the influence of N-glycosylation on its intracellular targeting Exp. Cell Res. . 315 , PubMedID: 19379732, DOI:
- Boeggeman, E., Ramakrishnan, B., Pasek, M., Manzoni, M., Puri, A., Loomis, K.H., Waybright, T.J., Qasba, P.K. 2009. Site specific conjugation of fluoroprobes to the remodeled Fc N-glycans of monoclonal antibodies using mutant glycosyltransferases: application for cell surface antigen detection Bioconjug. Chem. . 20 , PubMedID: 19425533, DOI:
- Rasmussen, T.N., Plenge, P., Bay, T., Egebjerg, J., Gether, U. 2009. A single nucleotide polymorphism in the human serotonin transporter introduces a new site for N-linked glycosylation Neuropharmacology . 57 , PubMedID: 19500602, DOI:
- Gong, B., Cukan, M., Fisher, R., Li, H., Stadheim, T.A., Gerngross, T. 2009. Characterization of N-linked glycosylation on recombinant glycoproteins produced in Pichia pastoris using ESI-MS and MALDI-TOF Methods Mol. Biol. . 534 , PubMedID: , DOI:
- Gefter, J.V., Shaufl, A.L., Fink, M.P., Delude, R.L. 2009. Comparison of distinct protein isoforms of the receptor for advanced glycation end-products expressed in murine tissues and cell lines Cell Tissue Res. . 337 , PubMedID: 19415334, DOI:
- Wagner-Rousset, E., Bednarczyk, A., Bussat, M.C., Colas, O., Corvaïa, N., Schaeffer, C., Van Dorsselaer, A., Beck, A. 2008. The way forward, enhanced characterization of therapeutic antibody glycosylation: comparison of three level mass spectrometry-based strategies J. Chromatogr. B Analyt. Technol. Biomed. Life Sci.. 872, PubMedID: 18672411, DOI:
- Graham, D.R., Mitsak, M.J., Elliott, S.T., Chen, D., Whelan, S.A., Hart, G.W., Van Eyk, J.E. 2008. Two-dimensional gel-based approaches for the assessment of N-Linked and O-GlcNAc glycosylation in human and simian immunodeficiency viruses Proteomics. 872, PubMedID: 19072736, DOI:
- Wong-Madden, S.T., Landry, D., and Guthrie, E.P. 1997. Discovery and Uses of Novel Glycosidases Techniques in Glycobiology. , PubMedID: , DOI:
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