LOCUS YEp24 7769 bp DNA circular 22-OCT-2007 DEFINITION Cloning vector YEp24, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector YEp24 ORGANISM Cloning vector YEp24 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 7769) AUTHORS New England Biolabs. TITLE Direct Submission JOURNAL Submitted (22-OCT-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA COMMENT See also GenBank accession L09156. FEATURES Location/Qualifiers source 1..7769 /organism="Cloning vector YEp24" /mol_type="other DNA" rep_origin 1..2247 /note="2 micron circle plasmid DNA; contains determinant for replication at high copy number in Saccharomyces cerevisiae" gene 2499..3302 /gene="URA3" CDS 2499..3302 /gene="URA3" /codon_start=1 /product="orotidine-5'-phosphate (OMP) decarboxylase" /translation="MSKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKEL LELVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVK LQYSAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLS TGEYTKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYR TVDDVVSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN" gene 3495..4685 /gene="tet" CDS 3495..4685 /gene="tet" /note="tetR (confers resistance to tetracycline)" /codon_start=1 /product="tetracycline efflux protein, class C" /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG QLQGSLAALTSLTSITGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR ATST" gene 5326..5517 /gene="rop" CDS 5326..5517 /gene="rop" /exception="alternative start codon" /codon_start=1 /transl_table=11 /product="ROP protein" /translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDH ADELYRSCLARFGDDGENL" rep_origin complement(5945..6533) /note="pMB1 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point)" gene complement(6704..7564) /gene="bla" CDS complement(6704..7564) /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" sig_peptide complement(7496..7564) /gene="bla" /note="required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein" BASE COUNT 2102 a 1830 c 1844 g 1993 t ORIGIN 1 gaattctgaa ccagtcctaa aacgagtaaa taggaccggc aattcttcaa gcaataaaca 61 ggaataccaa ttattaaaag ataacttagt cagatcgtac aataaagctt tgaagaaaaa 121 tgcgccttat tcaatctttg ctataaaaaa tggcccaaaa tctcacattg gaagacattt 181 gatgacctca tttctttcaa tgaagggcct aacggagttg actaatgttg tgggaaattg 241 gagcgataag cgtgcttctg ccgtggccag gacaacgtat actcatcaga taacagcaat 301 acctgatcac tacttcgcac tagtttctcg gtactatgca tatgatccaa tatcaaagga 361 aatgatagca ttgaaggatg agactaatcc aattgaggag tggcagcata tagaacagct 421 aaagggtagt gctgaaggaa gcatacgata ccccgcatgg aatgggataa tatcacagga 481 ggtactagac tacctttcat cctacataaa tagacgcata taagtacgca tttaagcata 541 aacacgcact atgccgttct tctcatgtat atatatatac aggcaacacg cagatatagg 601 tgcgacgtga acagtgagct gtatgtgcgc agctcgcgtt gcattttcgg aagcgctcgt 661 tttcggaaac gctttgaagt tcctattccg aagttcctat tctctagaaa gtataggaac 721 ttcagagcgc ttttgaaaac caaaagcgct ctgaagacgc actttcaaaa aaccaaaaac 781 gcaccggact gtaacgagct actaaaatat tgcgaatacc gcttccacaa acattgctca 841 aaagtatctc tttgctatat atctctgtgc tatatcccta tataacctac ccatccacct 901 ttcgctcctt gaacttgcat ctaaactcga cctctacatt ttttatgttt atctctagta 961 ttactcttta gacaaaaaaa ttgtagtaag aactattcat agagtgaatc gaaaacaata 1021 cgaaaatgta aacatttcct atacgtagta tatagagaca aaatagaaga aaccgttcat 1081 aattttctga ccaatgaaga atcatcaacg ctatcacttt ctgttcacaa agtatgcgca 1141 atccacatcg gtatagaata taatcgggga tgcctttatc ttgaaaaaat gcacccgcag 1201 cttcgctagt aatcagtaaa cgcgggaagt ggagtcaggc tttttttatg gaagagaaaa 1261 tagacaccaa agtagccttc ttctaacctt aacggaccta cagtgcaaaa agttatcaag 1321 agactgcatt atagagcgca caaaggagaa aaaaagtaat ctaagatgct ttgttagaaa 1381 aatagcgctc tcgggatgca tttttgtaga acaaaaaaga agtatagatt ctttgttggt 1441 aaaatagcgc tctcgcgttg catttctgtt ctgtaaaaat gcagctcaga ttctttgttt 1501 gaaaaattag cgctctcgcg ttgcattttt gttttacaaa aatgaagcac agattcttcg 1561 ttggtaaaat agcgctttcg cgttgcattt ctgttctgta aaaatgcagc tcagattctt 1621 tgtttgaaaa attagcgctc tcgcgttgca tttttgttct acaaaatgaa gcacagatgc 1681 ttcgttaaca aagatatgct attgaagtgc aagatggaaa cgcagaaaat gaaccgggga 1741 tgcgacgtgc aagattacct atgcaataga tgcaatagtt tctccaggaa ccgaaataca 1801 tacattgtct tccgtaaagc gctagactat atattattat acaggttcaa atatactatc 1861 tgtttcaggg aaaactccca ggttcggatg ttcaaaattc aatgatgggt aacaagtacg 1921 atcgtaaatc tgtaaaacag tttgtcggat attaggctgt atctcctcaa agcgtattcg 1981 aatatcattg agaagctgca gcgtcacatc ggataataat gatggcagcc attgtagaag 2041 tgccttttgc atttctagtc tctttctcgg tctagctagt tttactacat cgcgaagata 2101 gaatcttaga tcacactgcc tttgctgagc tggatcaata gagtaacaaa agagtggtaa 2161 ggcctcgtta aaggacaagg acctgagcgg aagtgtatcg tacagtagac ggagtatact 2221 agtatagtct atagtccgtg gaattctcat gtttgacagc ttatcatcga taagcttttc 2281 aattcaattc atcatttttt ttttattctt ttttttgatt tcggtttctt tgaaattttt 2341 ttgattcggt aatctccgaa cagaaggaag aacgaaggaa ggagcacaga cttagattgg 2401 tatatatacg catatgtagt gttgaagaaa catgaaattg cccagtattc ttaacccaac 2461 tgcacagaac aaaaacctgc aggaaacgaa gataaatcat gtcgaaagct acatataagg 2521 aacgtgctgc tactcatcct agtcctgttg ctgccaagct atttaatatc atgcacgaaa 2581 agcaaacaaa cttgtgtgct tcattggatg ttcgtaccac caaggaatta ctggagttag 2641 ttgaagcatt aggtcccaaa atttgtttac taaaaacaca tgtggatatc ttgactgatt 2701 tttccatgga gggcacagtt aagccgctaa aggcattatc cgccaagtac aattttttac 2761 tcttcgaaga cagaaaattt gctgacattg gtaatacagt caaattgcag tactctgcgg 2821 gtgtatacag aatagcagaa tgggcagaca ttacgaatgc acacggtgtg gtgggcccag 2881 gtattgttag cggtttgaag caggcggcag aagaagtaac aaaggaacct agaggccttt 2941 tgatgttagc agaattgtca tgcaagggct ccctatctac tggagaatat actaagggta 3001 ctgttgacat tgcgaagagc gacaaagatt ttgttatcgg ctttattgct caaagagaca 3061 tgggtggaag agatgaaggt tacgattggt tgattatgac acccggtgtg ggtttagatg 3121 acaagggaga cgcattgggt caacagtata gaaccgtgga tgatgtggtc tctacaggat 3181 ctgacattat tattgttgga agaggactat ttgcaaaggg aagggatgct aaggtagagg 3241 gtgaacgtta cagaaaagca ggctgggaag catatttgag aagatgcggc cagcaaaact 3301 aaaaaactgt attataagta aatgcatgta tactaaactc acaaattaga gcttcaattt 3361 aattatatca gttattaccc gggaatctcg gtcgtaatga tttttataat gacgaaaaaa 3421 aaaaaattgg aaagaaaaag ctttaatgcg gtagtttatc acagttaaat tgctaacgca 3481 gtcaggcacc gtgtatgaaa tctaacaatg cgctcatcgt catcctcggc accgtcaccc 3541 tggatgctgt aggcataggc ttggttatgc cggtactgcc gggcctcttg cgggatatcg 3601 tccattccga cagcatcgcc agtcactatg gcgtgctgct agcgctatat gcgttgatgc 3661 aatttctatg cgcacccgtt ctcggagcac tgtccgaccg ctttggccgc cgcccagtcc 3721 tgctcgcttc gctacttgga gccactatcg actacgcgat catggcgacc acacccgtcc 3781 tgtggatcct ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg 3841 ctggcgccta tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca 3901 tgagcgcttg tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg 3961 ccatctcctt gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac 4021 tgggctgctt cctaatgcag gagtcgcata agggagagcg tcgaccgatg cccttgagag 4081 ccttcaaccc agtcagctcc ttccggtggg cgcggggcat gactatcgtc gccgcactta 4141 tgactgtctt ctttatcatg caactcgtag gacaggtgcc ggcagcgctc tgggtcattt 4201 tcggcgagga ccgctttcgc tggagcgcga cgatgatcgg cctgtcgctt gcggtattcg 4261 gaatcttgca cgccctcgct caagccttcg tcactggtcc cgccaccaaa cgtttcggcg 4321 agaagcaggc cattatcgcc ggcatggcgg ccgacgcgct gggctacgtc ttgctggcgt 4381 tcgcgacgcg aggctggatg gccttcccca ttatgattct tctcgcttcc ggcggcatcg 4441 ggatgcccgc gttgcaggcc atgctgtcca ggcaggtaga tgacgaccat cagggacagc 4501 ttcaaggatc gctcgcggct cttaccagcc taacttcgat cactggaccg ctgatcgtca 4561 cggcgattta tgccgcctcg gcgagcacat ggaacgggtt ggcatggatt gtaggcgccg 4621 ccctatacct tgtctgcctc cccgcgttgc gtcgcggtgc atggagccgg gccacctcga 4681 cctgaatgga agccggcggc acctcgctaa cggattcacc actccaagaa ttggagccaa 4741 tcaattcttg cggagaactg tgaatgcgca aaccaaccct tggcagaaca tatccatcgc 4801 gtccgccatc tccagcagcc gcacgcggcg catctcgggc agcgttgggt cctggccacg 4861 ggtgcgcatg atcgtgctcc tgtcgttgag gacccggcta ggctggcggg gttgccttac 4921 tggttagcag aatgaatcac cgatacgcga gcgaacgtga agcgactgct gctgcaaaac 4981 gtctgcgacc tgagcaacaa catgaatggt cttcggtttc cgtgtttcgt aaagtctgga 5041 aacgcggaag tcagcgccct gcaccattat gttccggatc tgcatcgcag gatgctgctg 5101 gctaccctgt ggaacaccta catctgtatt aacgaagcgc tggcattgac cctgagtgat 5161 ttttctctgg tcccgccgca tccataccgc cagttgttta ccctcacaac gttccagtaa 5221 ccgggcatgt tcatcatcag taacccgtat cgtgagcatc ctctctcgtt tcatcggtat 5281 cattaccccc atgaacagaa attccccctt acacggaggc atcaagtgac caaacaggaa 5341 aaaaccgccc ttaacatggc ccgctttatc agaagccaga cattaacgct tctggagaaa 5401 ctcaacgagc tggacgcgga tgaacaggca gacatctgtg aatcgcttca cgaccacgct 5461 gatgagcttt accgcagctg cctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac 5521 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc 5581 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg cagccatgac ccagtcacgt 5641 agcgatagcg gagtgtatac tggcttaact atgcggcatc agagcagatt gtactgagag 5701 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc 5761 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 5821 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 5881 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 5941 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 6001 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 6061 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 6121 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 6181 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 6241 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 6301 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 6361 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 6421 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 6481 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 6541 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 6601 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 6661 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 6721 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 6781 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 6841 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 6901 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 6961 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg 7021 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 7081 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 7141 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 7201 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 7261 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 7321 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 7381 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 7441 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 7501 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 7561 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 7621 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 7681 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 7741 ggcgtatcac gaggcccttt cgtcttcaa //