LOCUS pSV40-CLuc 5256 bp DNA circular 29-APR-2010 DEFINITION Cloning vector pSV40-CLuc, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Control plasmid pSV40-CLuc ORGANISM Control plasmid pSV40-CLuc Unclassified. REFERENCE 1 (bases 1 to 5256) AUTHORS Nakajima,Y., Kobayashi,K., Yamagishi,K., Enomoto,T. and Ohmiya,Y. TITLE cDNA cloning and characterization of a secreted luciferase from the luminous Japanese ostracod, Cypridina noctiluca JOURNAL Biosci Biotechnol Biochem 68 (3), 565-570 (2004) PUBMED 15056888 REFERENCE 2 (bases 1 to 5256) AUTHORS Tzertzinis,G. TITLE Direct Submission JOURNAL Submitted (13-NOV-2009) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..5256 /organism="Control plasmid pSV40-CLuc" /mol_type="other DNA" misc_feature 1..50 /note="multiple cloning site (KpnI-HindIII)" promoter 51..246 /note="SV40 promoter (clockwise)" gene 291..1952 /gene="CLuc" CDS 291..1952 /gene="CLuc" /note="contains 30 codon substitutions for efficient mammalian translation" /codon_start=1 /product="Cypridina noctiluca luciferase" /translation="MKTLILAVALVYCATVHCQDCPYEPDPPNTVPTSCEAKEGECID SSCGTCTRDILSDGLCENKPGKTCCRMCQYVIECRVEAAGWFRTFYGKRFQFQEPGTY VLGQGTKGGDWKVSITLENLDGTKGAVLTKTRLEVAGDIIDIAQATENPITVNGGADP IIANPYTIGEVTIAVVEMPGFNITVIEFFKLIVIDILGGRSVRIAPDTANKGMISGLC GDLKMMEDTDFTSDPEQLAIQPKINQEFDGCPLYGNPDDVAYCKGLLEPYKDSCRNPI NFYYYTISCAFARCMGGDERASHVLLDYRETCAAPETRGTCVLSGHTFYDTFDKARYQ FQGPCKEILMAADCFWNTWDVKVSHRNVDSYTEVEKVRIRKQSTVVELIVDGKQILVG GEAVSVPYSSQNTSIYWQDGDILTTAILPEALVVKFNFKQLLVVHIRDPFDGKTCGIC GNYNQDFSDDSFDAEGACDLTPNPPGCTEEQKPEAERLCNSLFAGQSDLDQKCNVCHK PDRVERCMYEYCLRGQQGFCDHAWEFKKECYIKHGDTLEVPDECK" sig_peptide 291..344 /gene="CLuc" /note="General Secretory Pathway signal peptide" polyA_signal 1967..2188 /gene="CLuc" /note="SV40 polyA" enhancer 2195..2441 /gene="CLuc" /note="SV40 enhancer" rep_origin complement(2759..3347) /note="pUC19 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point)" gene complement(3518..4378) /gene="bla" CDS complement(3518..4378) /gene="bla" /note="ampR (confers resistance to ampicillin) (mutant V82I,A182V)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" rep_origin 4510..4955 /note="f1 origin of replication (+ -)" polyA_signal 5096..5255 /note="for background noise reduction" BASE COUNT 1365 a 1294 c 1269 g 1328 t ORIGIN 1 ggtaccaccg atcgatcgct agctgcagag ctcgaattcg atatcaagct tgcatctcaa 61 ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag 121 ttccgcccat tctccgcccc atcgctgact aatttttttt atttatgcag aggccgaggc 181 cgcctcggcc tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt 241 ttgcaactcg agattccggt actgttggta aactagtagg aggtagaacc atgaagacct 301 taattcttgc cgttgcatta gtctactgcg ccactgttca ttgccaggac tgtccttacg 361 aacctgatcc accaaacaca gttccaactt cctgtgaagc taaagaagga gaatgtattg 421 atagcagctg tggcacctgc acgagagaca tactatcaga tggactgtgt gaaaataaac 481 caggaaaaac atgttgccga atgtgtcagt atgtaattga atgcagagta gaggccgcag 541 gatggtttag aacattctat ggaaagagat tccagttcca ggaacctggt acatacgtgt 601 tgggtcaagg aaccaagggc ggcgactgga aggtgtccat caccctggag aacctggatg 661 gaaccaaggg ggctgtgctg accaagacaa gactggaagt ggctggagac atcattgaca 721 tcgctcaagc tactgagaat cccatcactg taaacggtgg agctgaccct atcatcgcca 781 acccgtacac catcggcgag gtcaccatcg ctgttgttga gatgccaggc ttcaacatca 841 ccgtcattga gttcttcaaa ctgatcgtga tcgacatcct cggaggaaga tctgtaagaa 901 tcgccccaga cacagcaaac aaaggaatga tctctggcct ctgtggagat cttaaaatga 961 tggaagatac agacttcact tcagatccag aacaactcgc tattcagcct aagatcaacc 1021 aggagtttga cggttgtcca ctctatggaa atcctgatga cgttgcatac tgcaaaggtc 1081 ttctggagcc gtacaaggac agctgccgca accccatcaa cttctactac tacaccatct 1141 cctgcgcctt cgcccgctgt atgggtggag acgagcgagc ctcacacgtg ctgcttgact 1201 acagggagac gtgcgctgct cccgaaacta gaggaacctg cgttttgtct ggacatactt 1261 tctacgatac atttgacaaa gcaagatacc aattccaggg tccctgcaag gagattctta 1321 tggccgccga ctgtttctgg aacacttggg atgtgaaggt ttcacacagg aatgttgact 1381 cttacactga agtagagaaa gtacgaatca ggaaacaatc gactgtagta gaactcattg 1441 ttgatggaaa acagattctg gttggaggag aagccgtgtc cgtcccgtac agctctcaga 1501 acacttccat ctactggcaa gatggtgaca tactgactac agccatccta cctgaagctc 1561 tggtggtcaa gttcaacttc aagcaactgc tcgtcgtaca tattagagat ccattcgatg 1621 gtaagacttg cggtatttgc ggtaactaca accaggattt cagtgatgat tcttttgatg 1681 ctgaaggagc ctgtgatctg acccccaacc caccgggatg caccgaagaa cagaaacctg 1741 aagctgaacg actctgcaat agtctcttcg ccggtcaaag tgatcttgat cagaaatgta 1801 acgtgtgcca caagcctgac cgtgtcgaac gatgcatgta cgagtattgc ctgaggggac 1861 aacagggttt ctgtgaccac gcatgggagt tcaagaaaga atgctacata aagcatggag 1921 acaccctaga agtaccagat gaatgcaaat agacgcgtac tctagacaga catgataaga 1981 tacattgatg agtttggaca aaccacaact agaatgcagt gaaaaaaatg ctttatttgt 2041 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac 2101 aacaacaatt gcattcattt tatgtttcag gttcaggggg aggtgtggga ggttttttaa 2161 agcaagtaaa acctctacaa atgtggtagg atccgataag gatctgaacg atggagcgga 2221 gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg cgggactatg 2281 gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga gcctggggac 2341 tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct gcctgctggg 2401 gagcctgggg actttccaca ccctaactga cacacattcc aggatccgtc gaccgatgcc 2461 cttgagagcc ttcaacccag tcagctcctt ccggtgggcg cggggcatga ctatcgtcgc 2521 cgcacttatg actgtcttct ttatcatgca actcgtagga caggtgccgg cagcgctctt 2581 ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag 2641 ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca 2701 tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt 2761 tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc 2821 gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct 2881 ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg 2941 tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca 3001 agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact 3061 atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta 3121 acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta 3181 actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct 3241 tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt 3301 tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga 3361 tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca 3421 tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat 3481 caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg 3541 cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt 3601 agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag 3661 acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc 3721 gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag 3781 ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca 3841 tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa 3901 ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga 3961 tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata 4021 attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca 4081 agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg 4141 ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg 4201 ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg 4261 cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag 4321 gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac 4381 tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca 4441 tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag 4501 tgccacctga cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca 4561 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct 4621 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt 4681 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac 4741 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct 4801 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt 4861 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac 4921 aaaaatttaa cgcgaatttt aacaaaatat taacgcttac aatttgccat tcgccattca 4981 ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagccca 5041 agctaccatg ataagtaagt aatattaagg tacgggaggt acttggagcg gccgcaataa 5101 aatatcttta ttttcattac atctgtgtgt tggttttttg tgtgaatcga tagtactaac 5161 atacgctctc catcaaaaca aaacgaaaca aaacaaacta gcaaaatagg ctgtccccag 5221 tgcaagtgca ggtgccagaa catttctcta tcgata //