LOCUS pSNAP-CaaX 5418 bp DNA circular 13-NOV-2009 DEFINITION Cloning vector pSNAP-CaaX, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pSNAP-CaaX ORGANISM Cloning vector pSNAP-CaaX other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5418) AUTHORS Brecht,A. and Muentner,K. TITLE Direct Submission JOURNAL Submitted (13-NOV-2009) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..5418 /organism="Cloning vector pSNAP-CaaX" /mol_type="other DNA" promoter 1..602 /note="CMV promoter region" gene 690..1274 /gene="SNAP26m" CDS 690..1274 /gene="SNAP26m" /note="SNAP26m sequence 690..1235, linker 1236..1259, CaaX box (farnesylation signal) sequence 1260..1271; SNAP26m optimized for mammalian expression" /codon_start=1 /product="O-6-methylguanine-DNA methyltransferase (C-terminal farnesylation signal)" /translation="MDKDCEMKRTTLDSPLGKLELSGCEQGLHEIKLLGKGTSAADAV EVPAPAAVLGGPEPLMQATAWLNAYFHQPEAIEEFPVPALHHPVFQQESFTRQVLWKL LKVVKFGEVISYQQLAALAGNPAATAAVKTALSGNPVPILIPCHRVVSSSGAVGGYEG GLAVKEWLLAHEGHRLGKPGLGPAGCMSCKCVLS" promoter complement(1327..1344) /note="T7 promoter" polyA_signal 1357..1740 /note="SV40 polyA" gene 1905..2765 /gene="bla" CDS 1905..2765 /gene="bla" /note="ampR (confers resistance to ampicillin) (mutant A182V)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" gene 3320..4114 /gene="aph(3')-II" CDS 3320..4114 /gene="aph(3')-II" /note="neoR, kanR, nptII (confers resistance to neomycin and kanamycin) (mutant R177S)" /codon_start=1 /product="aminoglycoside phosphotransferase from Tn5" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" rep_origin 4722..5310 /note="pUC19 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point)" BASE COUNT 1295 a 1413 c 1400 g 1310 t ORIGIN 1 atgcattagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga 61 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg 121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg 181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca 241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc 301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc 361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc 421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa 481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag 541 gcgtgtacgg tgggaggtct atataagcag agctggttta gtgaaccgtc agatccgcta 601 gcgattacgc caagctcgaa attaaccctc actaaaggga acaaaagctg gagctccacc 661 gcggtggcgg ccgcgatatc aagcttacca tggacaaaga ctgcgaaatg aagcgcacca 721 ccctggatag ccctctgggc aagctggaac tgtctgggtg cgaacagggc ctgcacgaga 781 tcaagctgct gggcaaagga acatctgccg ccgacgccgt ggaagtgcct gccccagccg 841 ccgtgctggg cggaccagag ccactgatgc aggccaccgc ctggctcaac gcctactttc 901 accagcctga ggccatcgag gagttccctg tgccagccct gcaccaccca gtgttccagc 961 aggagagctt tacccgccag gtgctgtgga aactgctgaa agtggtgaag ttcggagagg 1021 tcatcagcta ccagcagctg gccgccctgg ccggcaatcc cgccgccacc gccgccgtga 1081 aaaccgccct gagcggaaat cccgtgccca ttctgatccc ctgccaccgg gtggtgtcta 1141 gctctggcgc cgtggggggc tacgagggcg ggctcgccgt gaaagagtgg ctgctggccc 1201 acgagggcca cagactgggc aagcctgggc tgggtcctgc aggctgcatg agctgcaagt 1261 gtgtgctctc ctgaggatcc tgactcgagg gggggcccgg taccaggtaa gtgtacccaa 1321 ttcgccctat agtgagtcgt attacaattc actcgatcgc ccttcccaac agttgcgcag 1381 cctgaatggc gaatggagat ccaattttta agtgtataat gtgttaaact actgattcta 1441 attgtttgtg tattttagat tcacagtccc aaggctcatt tcaggcccct cagtcctcac 1501 agtctgttca tgatcataat cagccatacc acatttgtag aggttttact tgctttaaaa 1561 aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac 1621 ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat 1681 aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttaa 1741 cgcgtaaatt gtaagcgtta ataagcagaa ggccatcctg acggatggtc tttttgcgtt 1801 tctacaaact cttttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac 1861 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt 1921 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag 1981 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg 2041 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa 2101 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc 2161 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag 2221 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa 2281 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc 2341 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg 2401 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa 2461 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa 2521 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg 2581 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag 2641 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg 2701 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt 2761 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt 2821 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac 2881 gtgagttttc gttccactga gcgtcagaca ttgaaaaagg aagaatcctg aggcggaaag 2941 aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc 3001 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc 3061 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg 3121 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat 3181 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc 3241 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaagatcg atcaagagac 3301 aggatgagga tcgtttcgca tgattgaaca agatggattg cacgcaggtt ctccggccgc 3361 ttgggtggag aggctattcg gctatgactg ggcacaacag acaatcggct gctctgatgc 3421 cgccgtgttc cggctgtcag cgcaggggcg cccggttctt tttgtcaaga ccgacctgtc 3481 cggtgccctg aatgaactgc aagacgaggc agcgcggcta tcgtggctgg ccacgacggg 3541 cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg ggaagggact ggctgctatt 3601 gggcgaagtg ccggggcagg atctcctgtc atctcacctt gctcctgccg agaaagtatc 3661 catcatggct gatgcaatgc ggcggctgca tacgcttgat ccggctacct gcccattcga 3721 ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg atggaagccg gtcttgtcga 3781 tcaggatgat ctggacgaag aacatcaggg gctcgcgcca gccgaactgt tcgccaggct 3841 caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc catggcgatg cctgcttgcc 3901 gaatatcatg gtggaaaatg gccgcttttc tggattcatc gactgtggcc ggctgggtgt 3961 ggcggaccgc tatcaggaca tagcgttggc tacccgtgat attgctgaag aacttggcgg 4021 cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc gctcccgatt cgcagcgcat 4081 cgccttctat cgccttcttg acgagttctt ctgagcggga ctctggggtt cgaaatgacc 4141 gaccaagcga cgcccaacct gccatcacga gatttcgatt ccaccgccgc cttctatgaa 4201 aggttgggct tcggaatcgt tttccgggac gccggctgga tgatcctcca gcgcggggat 4261 ctcatgctgg agttcttcgc ccaccctagg gggaggctaa ctgaaacacg gaaggagaca 4321 ataccggaag gaacccgcgc tatgacggca ataaaaagac agaataaaac gcacggtgtt 4381 gggtcgtttg ttcataaacg cggggttcgg tcccagggct ggcactctgt cgatacccca 4441 ccgagacccc attggggcca atacgcccgc gtttcttcct tttccccacc ccacccccca 4501 agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg cggcaggccc tgccatagcc 4561 tcaggttact catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc 4621 taggtgaaga tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc 4681 cactgagcgt cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg 4741 cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg 4801 gatcaagagc taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca 4861 aatactgttc ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg 4921 cctacatacc tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg 4981 tgtcttaccg ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga 5041 acggggggtt cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac 5101 ctacagcgtg agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat 5161 ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc 5221 tggtatcttt atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga 5281 tgctcgtcag gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc 5341 ctggcctttt gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg 5401 gataaccgta ttaccgcc //