LOCUS pNEB193 2713 bp DNA circular 18-OCT-2007 DEFINITION Cloning vector pNEB193, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pNEB193 ORGANISM Cloning vector pNEB193 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 2713) AUTHORS Guan,C. TITLE Direct Submission JOURNAL Submitted (18-OCT-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..2713 /organism="Cloning vector pNEB193" /mol_type="other DNA" gene complement(146..496) /gene="lacZalpha" CDS complement(146..496) /gene="lacZalpha" /codon_start=1 /product="beta-galactosidase alpha fragment" /translation="MTMITPSLHACRFKQSTLDLIKDPARPRVPSSNSLAVVLQRRDW ENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWRLMRYFLLTHLCGISH RIWCTLSTICSDAA" misc_feature 396..479 /gene="lacZalpha" /note="multiple cloning site (EcoRI-HindIII)" -10_signal complement(541..546) /note="Plac promoter (drives lacZalpha; counter-clockwise); TATGTT" -35_signal complement(565..570) /note="Plac promoter (drives lacZalpha; counter-clockwise); TTTACA" protein_bind complement(590..602) /note="CAP protein binding site" /bound_moiety="catabolite activator protein (CAP)" rep_origin complement(894..1482) /note="pMB1 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point)" misc_RNA complement(894..1446) /note="RNAII transcript (complementary strand)" -35_signal 1300..1305 /note="RNAI promoter (clockwise); TTGAAG" -10_signal 1322..1327 /note="RNAI promoter (clockwise); GCTACA" misc_RNA 1336..1443 /note="RNAI transcript" -10_signal complement(1456..1461) /note="RNAII promoter (counter-clockwise); CGTAAT" -35_signal complement(1477..1482) /note="RNAII promoter (counter-clockwise); TTGAGA" gene complement(1653..2513) /gene="bla" CDS complement(1653..2513) /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" sig_peptide complement(2445..2513) /gene="bla" /note="required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein." -10_signal complement(2557..2562) /note="bla promoter (counter-clockwise); GAGACA; transcript start 2548 (complementary strand)" -35_signal complement(2578..2583) /note="bla promoter (counter-clockwise); TTCAAA" BASE COUNT 674 a 680 c 692 g 667 t ORIGIN 1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca 61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg 121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc 181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc 241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat 301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt 361 tttcccagtc acgacgttgt aaaacgacgg ccagtgaatt cgagctcggt acccgggggc 421 gcgccggatc cttaattaag tctagagtcg actgtttaaa cctgcaggca tgcaagcttg 481 gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac 541 aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc 601 acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg 661 cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct 721 tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac 781 tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga 841 gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat 901 aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac 961 ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct 1021 gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg 1081 ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg 1141 ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt 1201 cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg 1261 attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac 1321 ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga 1381 aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt 1441 gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt 1501 tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga 1561 ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc 1621 taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct 1681 atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata 1741 actacgatac gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca 1801 cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga 1861 agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga 1921 gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg 1981 gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga 2041 gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt 2101 gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct 2161 cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca 2221 ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat 2281 accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga 2341 aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc 2401 aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg 2461 caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc 2521 ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt 2581 gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca 2641 cctgacgtct aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg 2701 aggccctttc gtc //