LOCUS pGT-N38 4656 bp DNA circular 07-DEC-2007 DEFINITION Cloning vector pGT-N38, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pGT-N38 ORGANISM Cloning vector pGT-N38 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4656) AUTHORS Polakiewicz,R.D. TITLE Direct Submission JOURNAL Submitted (07-DEC-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..4656 /organism="Cloning vector pGT-N38" /mol_type="other DNA" gene 143..1003 /gene="bla" CDS 143..1003 /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERSPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" sig_peptide 143..211 /gene="bla" /note="Required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein." rep_origin complement(1045..1554) /note="M13 origin of replication (+ -)" rep_origin 1665..2253 /note="pMB1 origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point)" misc_feature 2465..2535 /note="multiple cloning site I (NotI-ApaI)" promoter 2526..3063 /note="phosphoglycerate kinase (PGK) promoter region" gene 3064..3867 /gene="aph(3')-II" CDS 3064..3867 /gene="aph(3')-II" /note="neoR, kanR, nptII (confers resistance to neomycin and kanamycin)" /codon_start=1 /product="aminoglycoside phosphotransferase from Tn5" /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSA QGRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPG QDLLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDD LDEEHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVA DRYQDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" polyA_signal 3868..4355 /note="PGK polyA" misc_feature 4351..4441 /note="multiple cloning site II (XbaI-AflII)" BASE COUNT 1096 a 1183 c 1242 g 1135 t ORIGIN 1 gttaactacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt 61 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca 121 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt 181 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga 241 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa 301 gatccttgag agttttcgcc ccgaagaacg ttctccaatg atgagcactt ttaaagttct 361 gctatgtggc gcggtattat cccgtgttga cgccgggcaa gagcaactcg gtcgccgcat 421 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga 481 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc 541 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat 601 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa 661 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac 721 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa 781 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc 841 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc 901 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag 961 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta 1021 ctcatatata ctttagattg atttaccccg gttgataatc agaaaagccc caaaaacagg 1081 aagattgtat aagcaaatat ttaaattgta aacgttaata ttttgttaaa attcgcgtta 1141 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat 1201 aaatcaaaag aatagcccga gatagggttg agtgttgttc cagtttggaa caagagtcca 1261 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc 1321 ccactacgtg aaccatcacc caaatcaagt tttttggggt cgaggtgccg taaagcacta 1381 aatcggaacc ctaaagggag cccccgattt agagcttgac ggggaaagcg aacgtggcga 1441 gaaaggaagg gaagaaagcg aaaggagcgg gcgctagggc gctggcaagt gtagcggtca 1501 cgctgcgcgt aaccaccaca cccgccgcgc ttaatgcgcc gctacagggc gcgtaaaagg 1561 atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg 1621 ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt 1681 ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg 1741 ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata 1801 ccaaatactg ttcttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca 1861 ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag 1921 tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc 1981 tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga 2041 tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg 2101 tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac 2161 gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg 2221 tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg 2281 ttcctggcct tttgctggcc ttttgctcac atgtaatgtg agttagctca ctcattaggc 2341 accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata 2401 acaatttcac acaggaaaca gctatgacca tgattacgcc aagctacgta atacgactca 2461 ctaggcggcc gcctagtggg gcccgtgcaa ttgaagccgg ctggcgccaa gcttctctgc 2521 aggattcgag ggcccctgca ggtcaattct accgggtagg ggaggcgctt ttcccaaggc 2581 agtctggagc atgcgcttta gcagccccgc tggcacttgg cgctacacaa gtggcctctg 2641 gcctcgcaca cattccacat ccaccggtag cgccaaccgg ctccgttctt tggtggcccc 2701 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg 2761 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc 2821 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc 2881 ttcgctttct gggctcagag gctgggaagg ggtgggtccg ggggcgggct caggggcggg 2941 ctcaggggcg gggcgggcgc gaaggtcctc ccgaggcccg gcattctcgc acgcttcaaa 3001 agcgcacgtc tgccgcgctg ttctcctctt cctcatctcc gggcctttcg acctgcagcc 3061 aatatgggat cggccattga acaagatgga ttgcacgcag gttctccggc cgcttgggtg 3121 gagaggctat tcggctatga ctgggcacaa cagacaatcg gctgctctga tgccgccgtg 3181 ttccggctgt cagcgcaggg gcgcccggtt ctttttgtca agaccgacct gtccggtgcc 3241 ctgaatgaac tgcaggacga ggcagcgcgg ctatcgtggc tggccacgac gggcgttcct 3301 tgcgcagctg tgctcgacgt tgtcactgaa gcgggaaggg actggctgct attgggcgaa 3361 gtgccggggc aggatctcct gtcatctcac cttgctcctg ccgagaaagt atccatcatg 3421 gctgatgcaa tgcggcggct gcatacgctt gatccggcta cctgcccatt cgaccaccaa 3481 gcgaaacatc gcatcgagcg agcacgtact cggatggaag ccggtcttgt cgatcaggat 3541 gatctggacg aagagcatca ggggctcgcg ccagccgaac tgttcgccag gctcaaggcg 3601 cgcatgcccg acggcgatga tctcgtcgtg acccatggcg atgcctgctt gccgaatatc 3661 atggtggaaa atggccgctt ttctggattc atcgactgtg gccggctggg tgtggcggac 3721 cgctatcagg acatagcgtt ggctacccgt gatattgctg aagagcttgg cggcgaatgg 3781 gctgaccgct tcctcgtgct ttacggtatc gccgctcccg attcgcagcg catcgccttc 3841 tatcgccttc ttgacgagtt cttctgaggg gatcgatccg tcctgtaagt ctgcagaaat 3901 tgatgatcta ttaaacaata aagatgtcca ctaaaatgga agtttttcct gtcatacttt 3961 gttaagaagg gtgagaacag agtacctaca ttttgaatgg aaggattgga gctacggggg 4021 tgggggtggg gtgggattag ataaatgcct gctctttact gaaggctctt tactattgct 4081 ttatgataat gtttcatagt tggatatcat aatttaaaca agcaaaacca aattaagggc 4141 cagctcattc ctcccactca tgatctatag atctatagat ctctcgtggg atcattgttt 4201 ttctcttgat tcccactttg tggttctaag tactgtggtt tccaaatgtg tcagtttcat 4261 agcctgaaga acgagatcag cagcctctgt tccacataca cttcattctc agtattgttt 4321 tgccaagttc taattccatc agaagctgac tctagatctg gatccacgaa ttcgctagct 4381 tcggccgtga cgcgtctccg gatgtacagg catgcgtcga ccctctagtc aaggccttaa 4441 gtgagtcgta ttacggactg gccgtcgttt tacaacgtcg tgactgggaa aaccctggcg 4501 ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt aatagcgaag 4561 aggcccgcac cgatcgccct tcccaacagt tgcgcagcct gaatggcgaa tggcgcttcg 4621 cttggtaata aagcccgctt cggcgggctt tttttt //