LOCUS pGPS1.1 4814 bp DNA circular 22-OCT-2007 DEFINITION Transposon donor vector pGPS1.1, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Transposon donor vector pGPS1.1 ORGANISM Transposon donor vector pGPS1.1 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 4814) AUTHORS Biery,M.C., Stewart,F.J., Stellwagen,A.E., Raleigh,E.A. and Craig,N.L. TITLE A simple in vitro Tn7-based transposition system with low target site selectivity for genome and gene analysis JOURNAL Nucleic Acids Res 28 (5), 1067-1077 (2000) PUBMED 10666445 REFERENCE 2 (bases 1 to 4814) AUTHORS Anton,B.P. TITLE Direct Submission JOURNAL Submitted (22-OCT-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..4814 /organism="Transposon donor vector pGPS1.1" /mol_type="other DNA" rep_origin complement(369..678) /note="R6K origin of replication (counter-clockwise)" rep_origin complement(732..1032) /note="R6K origin of replication (counter-clockwise)" gene 1416..2606 /gene="tet" CDS 1416..2606 /gene="tet" /note="tetR (confers resistance to tetracycline)" /codon_start=1 /product="tetracycline efflux protein, class C" /translation="MKSNNALIVILGTVTLDAVGIGLVMPVLPGLLRDIVHSDSIASH YGVLLALYALMQFLCAPVLGALSDRFGRRPVLLASLLGATIDYAIMATTPVLWILYAG RIVAGITGATGAVAGAYIADITDGEDRARHFGLMSACFGVGMVAGPVAGGLLGAISLH APFLAAAVLNGLNLLLGCFLMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTV FFIMQLVGQVPAALWVIFGEDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGE KQAIIAGMAADALGYVLLAFATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQG QLQGSLAALTSLTSIIGPLIVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSR ATST" misc_feature 3093..4791 /note="Transprimer-1 transposable element" misc_feature 3093..3290 /note="Tn7R (right end)" gene complement(3645..4460) /gene="aph(3')-Ia" CDS complement(3645..4460) /gene="aph(3')-Ia" /note="kanR (confers resistance to kanamycin)" /codon_start=1 /product="aminoglycoside phosphotransferase from Tn903" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" misc_feature 4625..4791 /note="Tn7L (left end)" BASE COUNT 1211 a 1209 c 1140 g 1254 t ORIGIN 1 ggtaccctgt gaatgcgcaa accaaccctt ggcagaacat atccatcgcg tccgccatct 61 ccagcagccg cacgcggcgc atctcgggca gcgttgggtc ctggccacgg gtgcgcatga 121 tcgtgctcct gtcgttgagg acccggctag gctggcgggg ttgccttact ggttagcaga 181 atgaatcacc gatacgcgag cgaacgtgaa gcgactgctg ctgcaaaacg tctgcgacct 241 gagcaacaac atgaatggtc ttcggtttcc gtgtttcgta aagtctggaa acgcggaagt 301 cagcgccctg caccattatg ttccggatct atgtcgggtg cggagaaaga ggtaatgaaa 361 tggcagatcc ctggcttgtt gtccacaacc gttaaacctt aaaagcttta aaagccttat 421 atattctttt ttttcttata aaacttaaaa ccttagaggc tatttaagtt gctgatttat 481 attaatttta ttgttcaaac atgagagctt agtacgtgaa acatgagagc ttagtacgtt 541 agccatgaga gcttagtacg ttagccatga gggtttagtt cgttaaacat gagagcttag 601 tacgttaaac atgagagctt agtacgtgaa acatgagagc ttagtacgta ctatcaacag 661 gttgaactgc tgatcttcgg atctatgtcg ggtgcggaga aagaggtaat gaaatggcag 721 atccctggct tgttgtccac aaccgttaaa ccttaaaagc tttaaaagcc ttatatattc 781 ttttttttct tataaaactt aaaaccttag aggctattta agttgctgat ttatattaat 841 tttattgttc aaacatgaga gcttagtacg tgaaacatga gagcttagta cgttagccat 901 gagagcttag tacgttagcc atgagggttt agttcgttaa acatgagagc ttagtacgtt 961 aaacatgaga gcttagtacg tgaaacatga gagcttagta cgtactatca acaggttgaa 1021 ctgctgatct tcggatctat gtcgggtgcg gagaaagagg taatgaaatg gcatccggat 1081 ctgcatcgca ggatgctgct ggctaccctg tggaacacct acatctgtat taacgaagca 1141 ttattgaagc atttatcagg gttattgtct catgagcgga tacatatttg aatgtattta 1201 gaaaaataaa caaatagggg ttccgcgcac atttccccga aaagtgccac ctgacgtcta 1261 agaaaccatt attatcatga cattaaccta taaaaatagg cgtatcacga ggccctttcg 1321 tcttcaagaa ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat 1381 cacagttaaa ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg 1441 tcatcctcgg caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc 1501 cgggcctctt gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc 1561 tagcgctata tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc 1621 gctttggccg ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga 1681 tcatggcgac cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca 1741 ccggcgccac aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc 1801 gggctcgcca cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg 1861 tggccggggg actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc 1921 tcaacggcct caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc 1981 gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca 2041 tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc 2101 cggcagcgct ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg 2161 gcctgtcgct tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc 2221 ccgccaccaa acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc 2281 tgggctacgt cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc 2341 ttctcgcttc cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag 2401 atgacgacca tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga 2461 tcattggacc gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt 2521 tggcatggat tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg 2581 catggagccg ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac 2641 cactccaaga attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc 2701 ttggcagaac atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg 2761 cagcgttggg tcctgggctg gcattgaccc tgagtgattt ttctctggtc ccgccgcatc 2821 cataccgcca gttgtttacc ctcacaacgt tccagtaacc gggcatgttc atcatcagta 2881 acccgtatcg tgagcatcct ctctcgtttc atcggtatca ttacccccat gaacagaaat 2941 cccccttaca cggaggcatc agtgaccaaa caggaaaaaa ccgcccttaa catggcccgc 3001 tttatcagaa gccagacatt aacgcttctg gagaaactca acgagctgga cgcggatgaa 3061 caggcagagc tcttactgtc atgccatccg tatgtgggcg gacaataaag tcttaaactg 3121 aacaaaatag atctaaacta tgacaataaa gtcttaaact agacagaata gttgtaaact 3181 gaaatcagtc cagttatgct gtgaaaaagc atactggact tttgttatgg ctaaagcaaa 3241 ctcttcattt tctgaagtgc aaattgcccg tcgtattaaa gaggggcgtg gggtcgacgc 3301 ggccgctaac tataacggtc ctaaggtagc gagtttaaac gatatcggat ccggccgccg 3361 ctgaggtctg cctcgtgaag aaggtgttgc tgactcatac caggcctgaa tcgccccatc 3421 atccagccag aaagtgaggg agccacggtt gatgagagct ttgttgtagg tggaccagtt 3481 ggtgattttg aacttttgct ttgccacgga acggtctgcg ttgtcgggaa gatgcgtgat 3541 ctgatccttc aactcagcaa gagttcgatt tattcaacaa agccgccgtc ccgtcaagtc 3601 agcgtaatgc tctgccagtg ttacaaccaa ttaaccaatt ctgattagaa aaactcatcg 3661 agcatcaaat gaaactgcaa tttattcata tcaggattat caataccata tttttgaaaa 3721 agccgtttct gtaatgaagg agaaaactca ccgaggcagt tccataggat ggcaagatcc 3781 tggtatcggt ctgcgattcc gactcgtcca acatcaatac aacctattaa tttcccctcg 3841 tcaaaaataa ggttatcaag tgagaaatca ccatgagtga cgactgaatc cggtgagaat 3901 ggcaaaagct tatgcatttc tttccagact tgttcaacag gccagccatt acgctcgtca 3961 tcaaaatcac tcgcatcaac caaaccgtta ttcattcgtg attgcgcctg agcgagacga 4021 aatacgcgat cgctgttaaa aggacaatta caaacaggaa tcgaatgcaa ccggcgcagg 4081 aacactgcca gcgcatcaac aatattttca cctgaatcag gatattcttc taatacctgg 4141 aatgctgttt tcccggggat cgcagtggtg agtaaccatg catcatcagg agtacggata 4201 aaatgcttga tggtcggaag aggcataaat tccgtcagcc agtttagtct gaccatctca 4261 tctgtaacat cattggcaac gctacctttg ccatgtttca gaaacaactc tggcgcatcg 4321 ggcttcccat acaatcgata gattgtcgca cctgattgcc cgacattatc gcgagcccat 4381 ttatacccat ataaatcagc atccatgttg gaatttaatc gcggcctcga gcaagacgtt 4441 tcccgttgaa tatggctcat aacacccctt gtattactgt ttatgtaagc agacagtttt 4501 attgttcatg atgatatatt tttatcttgt gcaatgtaac atcagagatt ttgagacaca 4561 acgtggctta ctaggatccg atatcattta aatctaggga taacagggta atactagtgt 4621 cgaccaacca gataagtgaa atctagttcc aaactatttt gtcattttta attttcgtat 4681 tagcttacga cgctacaccc agttcccatc tattttgtca ctcttcccta aataatcctt 4741 aaaaactcca tttccacccc tcccagttcc caactatttt gtccgcccac accgtaagat 4801 gcttttctgt gact //