LOCUS pGGAselect 2220 bp DNA circular UNA 17-JAN-2020 DEFINITION Cloning vector pGGAselect, complete sequence. ACCESSION urn.local...2l-bfa5soy VERSION urn.local...2l-bfa5soy KEYWORDS . SOURCE Cloning vector pGGAselect ORGANISM Cloning vector pGGAselect Unclassified. REFERENCE 1 (bases 1 to 2220) AUTHORS Kucera,R.B. TITLE Direct Submission JOURNAL Submitted (10-JAN-2020) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..2220 /dnas_title="pGGAselect" primer_bind 234..260 /note="GG assembly forward analysis primer sequence" regulatory 254..271 /regulatory_class="promoter" /note="SP6 promoter" /standard_name="SP6 promoter" misc_feature 284..301 /note="upstream multiple cloning site (XhoI-EcoRI)" misc_feature 315..318 /note="4 base BsaI overhang created during Golden Gate assembly (BsaI site 320-325)" /standard_name="BsaI overhang" misc_feature 319..401 /note="DNA sequence removed and replaced during Golden Gate assembly with BsaI" /standard_name="Fragment removed by BsaI" misc_feature 320..325 /standard_name="BsaI" misc_feature 326..329 /note="4 base BsmBI overhang created during Golden Gate assembly (BsmBI site 331-336)" /standard_name="BsmBI overhang" misc_feature 330..390 /note="DNA sequence removed and replaced during Golden Gate assembly with BsmBI" /standard_name="Fragment removed by BsmBI" misc_feature 331..336 /standard_name="BsmBI" misc_feature 337..340 /note="4 base BbsI overhang created during Golden Gate assembly (BbsI site 343-348)" /standard_name="BbsI overhang" misc_feature 341..379 /note="DNA sequence removed and replaced during Golden Gate assembly with BbsI" /standard_name="Fragment removed by BbsI" misc_feature 343..348 /standard_name="BbsI" misc_feature complement(372..377) /standard_name="BbsI" misc_feature complement(380..383) /note="4 base BbsI overhang created during Golden Gate assembly (BbsI site 372-377)" /standard_name="BbsI overhang" misc_feature complement(384..389) /standard_name="BsmBI" misc_feature complement(391..394) /note="4 base BsmBI overhang created during Golden Gate assembly (BsmBI site 384-389)" /standard_name="BsmBI overhang" misc_feature complement(395..400) /standard_name="BsaI" misc_feature complement(402..405) /note="4 base BsaI overhang created during Golden Gate assembly (BsaI site 395-400)" /standard_name="BsaI overhang" misc_feature 419..452 /note="downstream multiple cloning site (PacI-NotI)" primer_bind complement(457..482) /note="GG assembly reverse analysis primer sequence" regulatory complement(465..482) /regulatory_class="promoter" /note="T7 promoter" /standard_name="T7 promoter" CDS complement(593..1252) /gene="cat" /note="camR (confers resistance to chloramphenicol)" /codon_start=1 /product="chloramphenicol acetyltransferase" gene complement(593..1252) /gene="cat" rep_origin 1456..2044 ORIGIN 1 cgaaaaatca ataatcagac aacaagatgt gcgaactcga tattttacac gactctcttt 61 accaattctg ccccgaatta cacttaaaac gactcaacag cttaacgttg gcttgccacg 121 cattacttga ctgtaaaact ctcactctta ccgaacttgg ccgtaacctg ccaaccaaag 181 cgagaacaaa acataacatc aaacgaatcg accgattgtt aggtaatcgt cacctgcagg 241 aaggtttaaa cgcatttagg tgacactata gaagtgtgta tcgctcgagg gatccgaatt 301 cgaagtcttg gtacggagcg agaccggagc gagacgggag tcgtcttcgc tttccagatc 361 tgataacttg tgaagacgac catcgtctca ccatggtctc accattcctg tagacttctt 421 aattaagacg tcagaattct cgaggcggcc gcatgtgagt ctccctatag tgagtcgtat 481 taatttcgcg ggcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc 541 gctcatgagt agcaccaggc gtttaagggc accaataact gccttaaaaa aattacgccc 601 cgccctgcca ctcatcgcag tactgttgta attcattaag cattctgccg acatggaagc 661 catcacaaac ggcatgatga acctgaatcg ccagcggcat cagcaccttg tcgccttgcg 721 tataatattt gcccatggtg aaaacggggg cgaagaagtt gtccatattg gccacgttta 781 aatcaaaact ggtgaaactc acccagggat tggctgagac aaaaaacata ttctcaataa 841 accctttagg gaaataggcc aggttttcac cgtaacacgc cacatcttgc gaatatatgt 901 gtagaaactg ccggaaatcg tcgtggtatt cactccagag cgatgaaaac gtttcagttt 961 gctcatggaa aacggtgtaa caagggtgaa cactatccca tatcaccagc tcaccgtctt 1021 tcattgccat acgaaattcc ggatgagcat tcatcaggcg ggcaagaatg tgaataaagg 1081 ccggataaaa cttgtgctta tttttcttta cggtctttaa aaaggccgta atatccagct 1141 gaacggtctg gttataggta cattgagcaa ctgactgaaa tgcctcaaaa tgttctttac 1201 gatgccattg ggatatatca acggtggtat atccagtgat ttttttctcc attttagctt 1261 ccttagctcc tgaaaatctc gataactcaa aaaatacgcc cggtagtgat cttatttcat 1321 tatggtgaaa gttggaacct cttacgtgcc gatcaaagtc tcattttcgc caaaagttgt 1381 catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa 1441 gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa 1501 aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc 1561 gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag tgtagccgta 1621 gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct 1681 gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg 1741 atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag 1801 cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc 1861 cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg 1921 agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt 1981 tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg 2041 gaaaaacgcc agcaatgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca 2101 catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg cctttgagtg 2161 agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga gcgaggaagc //