LOCUS pET11c 5672 bp DNA circular 19-OCT-2007 DEFINITION Cloning vector pET11c, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pET11c ORGANISM Cloning vector pET11c other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 5672) AUTHORS New England Biolabs. TITLE Direct Submission JOURNAL Submitted (19-OCT-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..5672 /organism="Cloning vector pET11c" /mol_type="other DNA" gene complement(194..446) /gene="expression_cassette" terminator complement(194..316) /gene="expression_cassette" /note="T7 Tphi transcription terminator" misc_feature complement(356..358) /gene="expression_cassette" /note="translational start codon (complementary strand)" RBS complement(367..373) /gene="expression_cassette" /note="T7 phi10 RBS" protein_bind 403..427 /gene="expression_cassette" /note="lac operator" /bound_moiety="LacI" promoter complement(429..446) /gene="expression_cassette" /note="T7 phi10 promoter (transcript start 429 counter-clockwise)" gene 833..1915 /gene="lacI" CDS 833..1915 /gene="lacI" /exception="alternative start codon" /codon_start=1 /transl_table=11 /product="lac repressor (LacI)" /translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAE LNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERS GVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSII FSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREG DWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDT EDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNT QTASPRALADSLMQLARQVSRLESGQ" gene 3339..3530 /gene="rop" CDS 3339..3530 /gene="rop" /exception="alternative start codon" /codon_start=1 /transl_table=11 /product="ROP protein" /translation="MNRFTTTLMSFTAAASRVSVMTVKTSDTCSSRRRSQLVCKRMPG ADKPVRARQRVLAGVGAQP" rep_origin complement(3845..4433) /note="pMB1 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point)" gene complement(4604..5464) /gene="bla" CDS complement(4604..5464) /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" sig_peptide complement(5396..5464) /gene="bla" /note="required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein" BASE COUNT 1297 a 1549 c 1495 g 1331 t ORIGIN 1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa 61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg 121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt 181 gcgggatatc cggatatagt tcctcctttc agcaaaaaac ccctcaagac ccgtttagag 241 gccccaaggg gttatgctag ttattgctca gcggtggcag cagccaactc agcttccttt 301 cgggctttgt tagcagccgg atccgaccca tttgctgtcc accagtcatg ctagccatat 361 gtatatctcc ttcttaaagt taaacaaaat tatttctaga ggggaattgt tatccgctca 421 caattcccct atagtgagtc gtattaattt cgcgggatcg agatctcgat cctctacgcc 481 ggacgcatcg tggccggcat caccggcgcc acaggtgcgg ttgctggcgc ctatatcgcc 541 gacatcaccg atggggaaga tcgggctcgc cacttcgggc tcatgagcgc ttgtttcggc 601 gtgggtatgg tggcaggccc cgtggccggg ggactgttgg gcgccatctc cttgcatgca 661 ccattccttg cggcggcggt gctcaacggc ctcaacctac tactgggctg cttcctaatg 721 caggagtcgc ataagggaga gcgtcgagat cccggacacc atcgaatggc gcaaaacctt 781 tcgcggtatg gcatgatagc gcccggaaga gagtcaattc agggtggtga atgtgaaacc 841 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt 901 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc 961 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct 1021 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat 1081 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg 1141 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat 1201 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt 1261 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca 1321 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc 1381 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa 1441 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat 1501 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct 1561 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg 1621 cgttggtgcg gatatctcgg tagtgggata cgacgatacc gaagacagct catgttatat 1681 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg 1741 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact 1801 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc 1861 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgagcgca 1921 acgcaattaa tgtaagttag ctcactcatt aggcaccggg atctcgaccg atgcccttga 1981 gagccttcaa cccagtcagc tccttccggt gggcgcgggg catgactatc gtcgccgcac 2041 ttatgactgt cttctttatc atgcaactcg taggacaggt gccggcagcg ctctgggtca 2101 ttttcggcga ggaccgcttt cgctggagcg cgacgatgat cggcctgtcg cttgcggtat 2161 tcggaatctt gcacgccctc gctcaagcct tcgtcactgg tcccgccacc aaacgtttcg 2221 gcgagaagca ggccattatc gccggcatgg cggccgacgc gctgggctac gtcttgctgg 2281 cgttcgcgac gcgaggctgg atggccttcc ccattatgat tcttctcgct tccggcggca 2341 tcgggatgcc cgcgttgcag gccatgctgt ccaggcaggt agatgacgac catcagggac 2401 agcttcaagg atcgctcgcg gctcttacca gcctaacttc gatcactgga ccgctgatcg 2461 tcacggcgat ttatgccgcc tcggcgagca catggaacgg gttggcatgg attgtaggcg 2521 ccgccctata ccttgtctgc ctccccgcgt tgcgtcgcgg tgcatggagc cgggccacct 2581 cgacctgaat ggaagccggc ggcacctcgc taacggattc accactccaa gaattggagc 2641 caatcaattc ttgcggagaa ctgtgaatgc gcaaaccaac ccttggcaga acatatccat 2701 cgcgtccgcc atctccagca gccgcacgcg gcgcatctcg ggcagcgttg ggtcctggcc 2761 acgggtgcgc atgatcgtgc tcctgtcgtt gaggacccgg ctaggctggc ggggttgcct 2821 tactggttag cagaatgaat caccgatacg cgagcgaacg tgaagcgact gctgctgcaa 2881 aacgtctgcg acctgagcaa caacatgaat ggtcttcggt ttccgtgttt cgtaaagtct 2941 ggaaacgcgg aagtcagcgc cctgcaccat tatgttccgg atctgcatcg caggatgctg 3001 ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt gaccctgagt 3061 gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac aacgttccag 3121 taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc gtttcatcgg 3181 tatcattacc cccatgaaca gaaatccccc ttacacggag gcatcagtga ccaaacagga 3241 aaaaaccgcc cttaacatgg cccgctttat cagaagccag acattaacgc ttctggagaa 3301 actcaacgag ctggacgcgg atgaacaggc agacatctgt gaatcgcttc acgaccacgc 3361 tgatgagctt taccgcagct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca 3421 catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc 3481 ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg 3541 tagcgatagc ggagtgtata ctggcttaac tatgcggcat cagagcagat tgtactgaga 3601 gtgcaccatt gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc 3661 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg 3721 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa 3781 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg 3841 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga 3901 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg 3961 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg 4021 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc 4081 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg 4141 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca 4201 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt 4261 ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag 4321 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg 4381 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc 4441 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt 4501 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt 4561 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca 4621 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg 4681 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac 4741 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg 4801 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc 4861 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgctg 4921 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac 4981 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc 5041 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac 5101 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact 5161 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa 5221 cacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt 5281 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca 5341 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa 5401 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac 5461 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg 5521 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc 5581 gaaaagtgcc acctgacgtc taagaaacca ttattatcat gacattaacc tataaaaata 5641 ggcgtatcac gaggcccttt cgtcttcaag aa //