LOCUS pCMV-GLuc 5764 bp DNA circular 19-OCT-2007 DEFINITION Control vector pCMV-GLuc, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Control vector pCMV-GLuc ORGANISM Control vector pCMV-GLuc Unclassified. REFERENCE 1 (bases 1 to 5764) AUTHORS Tzertzinis,G. TITLE Direct Submission JOURNAL Submitted (19-OCT-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..5764 /organism="Control vector pCMV-GLuc" /mol_type="other DNA" promoter 209..863 /note="CMV promoter (clockwise)" gene 920..1477 /gene="GLuc" CDS 920..1477 /gene="GLuc" /codon_start=1 /product="Gaussia princeps luciferase" /translation="MGVKVLFALICIAVAEAKPTENNEDFNIVAVASNFATTDLDADR GKLPGKKLPLEVLKEMEANARKAGCTRGCLICLSHIKCTPKMKKFIPGRCHTYEGDKE SAQGGIGEAIVDIPEIPGFKDLEPMEQFIAQVDLCVDCTTGCLKGLANVQCSDLLKKW LPQRCATFASKIQGQVDKIKGAGGD" sig_peptide 920..970 /gene="GLuc" /note="secretory signal sequence" promoter 2082..2417 /note="SV40 promoter (clockwise)" gene 2469..3263 /gene="aph(3')-II" CDS 2469..3263 /gene="aph(3')-II" /note="neoR, kanR, nptII (confers resistance to neomycin and kanamycin)" /codon_start=1 /product="aminoglycoside phosphotransferase from Tn5" /translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGR PVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDL LSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRY QDIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF" rep_origin complement(4009..4597) /note="pMB1 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point)" gene complement(4768..5628) /gene="bla" CDS complement(4768..5628) /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" BASE COUNT 1340 a 1510 c 1490 g 1424 t ORIGIN 1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg 61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg 121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc 181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt 241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata 301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc 361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc 421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt 481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt 541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca 601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg 661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc 721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg 781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca 841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc 901 gagctcggat ccagccacca tgggagtcaa agttctgttt gccctgatct gcatcgctgt 961 ggccgaggcc aagcccaccg agaacaacga agacttcaac atcgtggccg tggccagcaa 1021 cttcgcgacc acggatctcg atgctgaccg cgggaagttg cccggcaaga agctgccgct 1081 ggaggtgctc aaagagatgg aagccaatgc ccggaaagct ggctgcacca ggggctgtct 1141 gatctgcctg tcccacatca agtgcacgcc caagatgaag aagttcatcc caggacgctg 1201 ccacacctac gaaggcgaca aagagtccgc acagggcggc ataggcgagg cgatcgtcga 1261 cattcctgag attcctgggt tcaaggactt ggagcccatg gagcagttca tcgcacaggt 1321 cgatctgtgt gtggactgca caactggctg cctcaaaggg cttgccaacg tgcagtgttc 1381 tgacctgctc aagaagtggc tgccgcaacg ctgtgcgacc tttgccagca agatccaggg 1441 ccaggtggac aagatcaagg gggccggtgg tgactaagcg gccgctcgag catgcatcta 1501 gaaataattc ttactgtcat gccaagtaag atgcttttct gtgctgcaat agcaggcatg 1561 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg 1621 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca 1681 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct 1741 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcggggcatc cctttagggt 1801 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac 1861 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct 1921 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt 1981 ttgatttata agggattttg gggatttcgg cctattggtt aaaaaatgag ctgatttaac 2041 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc 2101 aggctcccca ggcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt 2161 gtggaaagtc cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt 2221 cagcaaccat agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg 2281 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct 2341 ctgcctctga gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca 2401 aaaagctccc gggagcttgt atatccattt tcggatctga tcaagagaca ggatgaggat 2461 cgtttcgcat gattgaacaa gatggattgc acgcaggttc tccggccgct tgggtggaga 2521 ggctattcgg ctatgactgg gcacaacaga caatcggctg ctctgatgcc gccgtgttcc 2581 ggctgtcagc gcaggggcgc ccggttcttt ttgtcaagac cgacctgtcc ggtgccctga 2641 atgaactgca ggacgaggca gcgcggctat cgtggctggc cacgacgggc gttccttgcg 2701 cagctgtgct cgacgttgtc actgaagcgg gaagggactg gctgctattg ggcgaagtgc 2761 cggggcagga tctcctgtca tctcaccttg ctcctgccga gaaagtatcc atcatggctg 2821 atgcaatgcg gcggctgcat acgcttgatc cggctacctg cccattcgac caccaagcga 2881 aacatcgcat cgagcgagca cgtactcgga tggaagccgg tcttgtcgat caggatgatc 2941 tggacgaaga gcatcagggg ctcgcgccag ccgaactgtt cgccaggctc aaggcgcgca 3001 tgcccgacgg cgaggatctc gtcgtgaccc atggcgatgc ctgcttgccg aatatcatgg 3061 tggaaaatgg ccgcttttct ggattcatcg actgtggccg gctgggtgtg gcggaccgct 3121 atcaggacat agcgttggct acccgtgata ttgctgaaga gcttggcggc gaatgggctg 3181 accgcttcct cgtgctttac ggtatcgccg ctcccgattc gcagcgcatc gccttctatc 3241 gccttcttga cgagttcttc tgagcgggac tctggggttc gaaatgaccg accaagcgac 3301 gcccaacctg ccatcacgag atttcgattc caccgccgcc ttctatgaaa ggttgggctt 3361 cggaatcgtt ttccgggacg ccggctggat gatcctccag cgcggggatc tcatgctgga 3421 gttcttcgcc caccccaact tgtttattgc agcttataat ggttacaaat aaagcaatag 3481 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa 3541 actcatcaat gtatcttatc atgtctgtat accgtcgacc tctagctaga gcttggcgta 3601 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat 3661 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt 3721 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta 3781 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc 3841 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa 3901 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa 3961 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct 4021 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac 4081 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc 4141 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc 4201 tcaatgctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg 4261 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga 4321 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag 4381 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta 4441 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag 4501 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg 4561 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac 4621 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc 4681 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag 4741 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc 4801 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac 4861 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc 4921 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg 4981 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag 5041 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc 5101 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac 5161 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag 5221 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac 5281 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg 5341 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc 5401 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact 5461 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg 5521 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa 5581 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt 5641 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg 5701 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga 5761 cgtc //