LOCUS pACYC177 3941 bp DNA circular 19-OCT-2007 DEFINITION Cloning vector pACYC177, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Cloning vector pACYC177 ORGANISM Cloning vector pACYC177 other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 3941) AUTHORS New England Biolabs. TITLE Direct Submission JOURNAL Submitted (19-OCT-2007) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA COMMENT See also GenBank accession X06402. FEATURES Location/Qualifiers source 1..3941 /organism="Cloning vector pACYC177" /mol_type="other DNA" gene join(3699..3941,1..618) /gene="bla" CDS join(3699..3941,1..618) /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" sig_peptide 3699..3767 /gene="bla" /note="required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein." rep_origin 783..1328 /note="p15A origin of replication (clockwise) (RNAII -35 to RNA/DNA switch point)" gene 1923..2738 /gene="aph(3')-Ia" CDS 1923..2738 /gene="aph(3')-Ia" /note="kanR (confers resistance to kanamycin)" /codon_start=1 /product="aminoglycoside phosphotransferase from Tn903" /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF" BASE COUNT 1026 a 893 c 993 g 1029 t ORIGIN 1 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt 61 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc 121 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga 181 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat 241 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct 301 gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc 361 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg 421 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc 481 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg 541 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca 601 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta 661 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc 721 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccttaat aagatgatct 781 tcttgagatc gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg 841 cagggcggtt tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt 901 ggaggagcgc agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc 961 aagactaact cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct 1021 ttccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg 1081 gggttcgtgc atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg 1141 tggaatgaga caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa 1201 caggagagcg cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg 1261 ggtttcgcca ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg gggcggagcc 1321 tatggaaaaa cggctttgcc gcggccctct cacttccctg ttaagtatct tcctggcatc 1381 ttccaggaaa tctccgcccc gttcgtaagc catttccgct cgccgcagtc gaacgaccga 1441 gcgtagcgag tcagtgagcg aggaagcgga atatatcctg tatcacatat tctgctgacg 1501 caccggtgca gccttttttc tcctgccaca tgaagcactt cactgacacc ctcatcagtg 1561 ccaacatagt aagccagtat acactccgct agcgctgagg tctgcctcgt gaagaaggtg 1621 ttgctgactc ataccaggcc tgaatcgccc catcatccag ccagaaagtg agggagccac 1681 ggttgatgag agctttgttg taggtggacc agttggtgat tttgaacttt tgctttgcca 1741 cggaacggtc tgcgttgtcg ggaagatgcg tgatctgatc cttcaactca gcaaaagttc 1801 gatttattca acaaagccac gttgtgtctc aaaatctctg atgttacatt gcacaagata 1861 aaaatatatc atcatgaaca ataaaactgt ctgcttacat aaacagtaat acaaggggtg 1921 ttatgagcca tattcaacgg gaaacgtctt gctcgaggcc gcgattaaat tccaacatgg 1981 atgctgattt atatgggtat aaatgggctc gcgataatgt cgggcaatca ggtgcgacaa 2041 tctatcgatt gtatgggaag cccgatgcgc cagagttgtt tctgaaacat ggcaaaggta 2101 gcgttgccaa tgatgttaca gatgagatgg tcagactaaa ctggctgacg gaatttatgc 2161 ctcttccgac catcaagcat tttatccgta ctcctgatga tgcatggtta ctcaccactg 2221 cgatccccgg gaaaacagca ttccaggtat tagaagaata tcctgattca ggtgaaaata 2281 ttgttgatgc gctggcagtg ttcctgcgcc ggttgcattc gattcctgtt tgtaattgtc 2341 cttttaacag cgatcgcgta tttcgtctcg ctcaggcgca atcacgaatg aataacggtt 2401 tggttgatgc gagtgatttt gatgacgagc gtaatggctg gcctgttgaa caagtctgga 2461 aagaaatgca taagcttttg ccattctcac cggattcagt cgtcactcat ggtgatttct 2521 cacttgataa ccttattttt gacgagggga aattaatagg ttgtattgat gttggacgag 2581 tcggaatcgc agaccgatac caggatcttg ccatcctatg gaactgcctc ggtgagtttt 2641 ctccttcatt acagaaacgg ctttttcaaa aatatggtat tgataatcct gatatgaata 2701 aattgcagtt tcatttgatg ctcgatgagt ttttctaatc agaattggtt aattggttgt 2761 aacactggca gagcattacg ctgacttgac gggacggcgg ctttgttgaa taaatcgaac 2821 ttttgctgag ttgaaggatc agatcacgca tcttcccgac aacgcagacc gttccgtggc 2881 aaagcaaaag ttcaaaatca ccaactggtc cacctacaac aaagctctca tcaaccgtgg 2941 ctccctcact ttctggctgg atgatggggc gattcaggcc tggtatgagt cagcaacacc 3001 ttcttcacga ggcagacctc agcgctcaaa gatgcagggg taaaagctaa ccgcatcttt 3061 accgacaagg catccggcag ttcaacagat cgggaagggc tggatttgct gaggatgaag 3121 gtggaggaag gtgatgtcat tctggtgaag aagctcgacc gtcttggccg cgacaccgcc 3181 gacatgatcc aactgataaa agagtttgat gctcagggtg tagcggttcg gtttattgac 3241 gacgggatca gtaccgacgg tgatatgggg caaatggtgg tcaccatcct gtcggctgtg 3301 gcacaggctg aacgccggag gatcctagag cgcacgaatg agggccgaca ggaagcaaag 3361 ctgaaaggaa tcaaatttgg ccgcaggcgt accgtggaca ggaacgtcgt gctgacgctt 3421 catcagaagg gcactggtgc aacggaaatt gctcatcagc tcagtattgc ccgctccacg 3481 gtttataaaa ttcttgaaga cgaaagggcc tcgtgatacg cctattttta taggttaatg 3541 tcatgataat aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa 3601 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac 3661 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg 3721 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc 3781 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg 3841 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga 3901 gcacttttaa agttctgcta tgtggcgcgg tattatcccg t //