LOCUS       N-His                   2775 bp    DNA     linear       21-FEB-
2020
FEATURES             Location/Qualifiers
     misc_feature    88..615
                     /note="DHFR"
     misc_feature    772..813
                     /note=""
     misc_feature    1..21
                     /note="pUCA105T7_F primer"
     misc_feature    26..42
                     /note="T7 promoter"
     misc_feature    88..135
                     /note="N-His"
     source          1..2775
                     /dnas_title="N-His DHFR UAA (NEB) plasmid"
ORIGIN      
        1 GCTAGTGGTG CTAGCCCCGC GAAATTAATA CGACTCACTA TAGGGTCTAG AAATAATTTT
       61 GTTTAACTTT AAGAAGGAGA TATACATatg AAAATCGAAG AAGGTAAAGG TCACCATCAC
      121 CATCACCACg gatccATGAT CAGTCTGATT GCGGCGTTAG CGGTAGATCG CGTTATCGGC
      181 ATGGAAAACG CCATGCCGTG GAACCTGCCT GCCGATCTCG CCTGGTTTAA ACGCAACACC
      241 TTAAATAAAC CCGTGATTAT GGGCCGCCAT ACCTGGGAAT CAATCGGTCG TCCGTTGCCA
      301 GGACGCAAAA ATATTATCCT CAGCAGTCAA CCGGGTACGG ACGATCGCGT AACGTGGGTG
      361 AAGTCGGTGG ATGAAGCCAT CGCGGCGTGT GGTGACGTAC CAGAAATCAT GGTGATTGGC
      421 GGCGGTCGCG TTTATGAACA GTTCTTGCCA AAAGCGCAAA AACTGTATCT GACGCATATC
      481 GACGCAGAAG TGGAAGGCGA CACCCATTTC CCGGATTACG AGCCGGATGA CTGGGAATCG
      541 GTATTCAGCG AATTCCACGA TGCTGATGCG CAGAACTCTC ACAGCTATTG CTTTGAGATT
      601 CTGGAGCGGC GGTAAGGAGG ATCCCGGGAA TTCTCGAGTA AGGTTAACCT GCAGGAGGCC
      661 TTTAATTAAG GTGGTGCGGC CGCGCTAGCG GTCCCGGGGG ATCGATCCGG CTGCTAACAA
      721 AGCCCGAAAG GAAGCTGAGT TGGCTGCTGC CACCGCTGAG CAATAACTAG CATAACCCCT
      781 TGGGGCCTCT AAACGGGTCT TGAGGGGTTT TTTGCTGAAA GGAGGAACTA TATCCGGAAG
      841 CTTGGCACTG GCCGACCGGG GTCGAGCACT GACTCGCTGC GCTCGGTCGT TCGGCTGCGG
      901 CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC
      961 GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG
     1021 TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA
     1081 AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC
     1141 TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC
     1201 CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG
     1261 GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC
     1321 TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGCTAAGAC ACGACTTATC GCCACTGGCA
     1381 GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG
     1441 AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG
     1501 AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT
     1561 GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA
     1621 GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACAGATC
     1681 CGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA
     1741 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAAACT TGGTCTGACA GTTACCAATG
     1801 CTTAATCAGT GAGGCACCTA TCTCAGCGAT CTGTCTATTT CGTTCATCCA TAGTTGCCTG
     1861 ACTCCCCGTC GTGTAGATAA CTACGATACG GGAGGGCTTA CCATCTGGCC CCAGTGCTGC
     1921 AATGATACCG CGAGACCCAC GCTCACCGGC TCCAGATTTA TCAGCAATAA ACCAGCCAGC
     1981 CGGAAGGGCC GAGCGCAGAA GTGGTCCTGC AACTTTATCC GCCTCCATCC AGTCTATTAA
     2041 TTGTTGCCGG GAAGCTAGAG TAAGTAGTTC GCCAGTTAAT AGTTTGCGCA ACGTTGTTGC
     2101 CATTGCTACA GGCATCGTGG TGTCACGCTC GTCGTTTGGT ATGGCTTCAT TCAGCTCCGG
     2161 TTCCCAACGA TCAAGGCGAG TTACATGATC CCCCATGTTG TGCAAAAAAG CGGTTAGCTC
     2221 CTTCGGTCCT CCGATCGTTG TCAGAAGTAA GTTGGCCGCA GTGTTATCAC TCATGGTTAT
     2281 GGCAGCACTG CATAATTCTC TTACTGTCAT GCCATCCGTA AGATGCTTTT CTGTGACTGG
     2341 TGAGTACTCA ACCAAGTCAT TCTGAGAATA GTGTATGCGG CGACCGAGTT GCTCTTGCCC
     2401 GGCGTCAATA CGGGATAATA CCGCGCCACA TAGCAGAACT TTAAAAGTGC TCATCATTGG
     2461 AAAACGTTCT TCGGGGCGAA AACTCTCAAG GATCTTACCG CTGTTGAGAT CCAGTTCGAT
     2521 GTAACCCACT CGTGCACCCA ACTGATCTTC AGCATCTTTT ACTTTCACCA GCGTTTCTGG
     2581 GTGAGCAAAA ACAGGAAGGC AAAATGCCGC AAAAAAGGGA ATAAGGGCGA CACGGAAATG
     2641 TTGAATACTC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT
     2701 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC
     2761 ATTTCCCCGA AAAGT
//