LOCUS DHFR_Control_Template 2727 bp DNA circular 30-DEC-2009 DEFINITION Control vector DHFR_Control_Template, complete sequence. ACCESSION VERSION KEYWORDS . SOURCE Control vector DHFR_Control_Template ORGANISM Control vector DHFR_Control_Template other sequences; artificial sequences; vectors. REFERENCE 1 (bases 1 to 2727) AUTHORS Cantor,E. TITLE Direct Submission JOURNAL Submitted (30-DEC-2009) Research Department, New England Biolabs, 240 County Road, Ipswich, MA 01938, USA FEATURES Location/Qualifiers source 1..2727 /organism="Control vector DHFR_Control_Template" /mol_type="other DNA" promoter 26..43 /note="T7 promoter (transcript start 43 clockwise)" gene 88..567 /gene="folA" CDS 88..567 /gene="folA" /codon_start=1 /product="dihydrofolate reductase (DHFR)" /translation="MISLIAALAVDRVIGMENAMPWNLPADLAWFKRNTLNKPVIMGR HTWESIGRPLPGRKNIILSSQPGTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVYEQ FLPKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR" terminator 663..785 /note="T7 Tphi transcription terminator" rep_origin complement(984..1572) /note="pUC19 origin of replication (counter-clockwise) (RNAII -35 to RNA/DNA switch point)" gene complement(1744..2604) /gene="bla" CDS complement(1744..2604) /gene="bla" /note="ampR (confers resistance to ampicillin)" /codon_start=1 /product="beta-lactamase" /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVE YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA EIGASLIKHW" sig_peptide complement(2536..2604) /gene="bla" /note="Required for secretion to the periplasm; cleaved off to form the mature beta-lactamase protein." BASE COUNT 694 a 671 c 694 g 668 t ORIGIN 1 gctagtggtg ctagccccgc gaaattaata cgactcacta tagggtctag aaataatttt 61 gtttaacttt aagaaggaga tatacatatg atcagtctga ttgcggcgtt agcggtagat 121 cgcgttatcg gcatggaaaa cgccatgccg tggaacctgc ctgccgatct cgcctggttt 181 aaacgcaaca ccttaaataa acccgtgatt atgggccgcc atacctggga atcaatcggt 241 cgtccgttgc caggacgcaa aaatattatc ctcagcagtc aaccgggtac ggacgatcgc 301 gtaacgtggg tgaagtcggt ggatgaagcc atcgcggcgt gtggtgacgt accagaaatc 361 atggtgattg gcggcggtcg cgtttatgaa cagttcttgc caaaagcgca aaaactgtat 421 ctgacgcata tcgacgcaga agtggaaggc gacacccatt tcccggatta cgagccggat 481 gactgggaat cggtattcag cgaattccac gatgctgatg cgcagaactc tcacagctat 541 tgctttgaga ttctggagcg gcggtaatga ggatcccggg aattctcgag taaggttaac 601 ctgcaggagg cctttaatta aggtggtgcg gccgcgctag cggtcccggg ggatcgatcc 661 ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg agcaataact 721 agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga aaggaggaac 781 tatatccgga agcttggcac tggccgaccg gggtcgagca ctgactcgct gcgctcggtc 841 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa 901 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt 961 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa 1021 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt 1081 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg 1141 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc 1201 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc 1261 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccgctaag acacgactta 1321 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct 1381 acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt atttggtatc 1441 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa 1501 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa 1561 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa 1621 aactcacaga tccgggattt tggtcatgag attatcaaaa aggatcttca cctagatcct 1681 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga 1741 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc 1801 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg 1861 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat 1921 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat 1981 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg 2041 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc 2101 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa 2161 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc 2221 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt 2281 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag 2341 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt 2401 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag 2461 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac 2521 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc 2581 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca 2641 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg 2701 ggttccgcgc acatttcccc gaaaagt //